Single-channel microchip for fast screening and detailed identification of nitroaromatic explosives or organophosphate nerve agents
Single-channel microchip for fast screening and detailed identification of nitroaromatic explosives or organophosphate nerve agents
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DOI：
10.1021/ac0111356
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发表时间：
2002-03-01
影响因子：
7.4
通讯作者：
Olsen, K
中科院分区：
文献类型：
--
作者：
Wang, J;Pumera, M;Olsen, K
描述了一种基于单通道芯片的分析微系统，该系统允许快速流动注射测量有机炸药或神经毒剂化合物的总含量，以及详细的单个化合物的胶束色谱鉴定。该协议涉及重复快速流动注射（筛选）分析，以提供及时的警告和警报，只有当检测到有害化合物时才切换到分离（指纹识别）模式。在十二烷基硫酸钠（SDS）存在下，胶束电动色谱法用于分离中性硝基芳族炸药和神经毒剂化合物，而在没有SDS的情况下进行操作，则可高速测量“总”炸药或神经毒剂含量。通过在分离通道中快速交换无SDS和含SDS的缓冲液来实现“流动注射”和“分离”模式之间的切换。安培检测用于监测分离。对影响样品通量、分辨率和灵敏度的关键因素进行了评估和优化。因此，对于“总”筛选和“个体”测量，可以分别实现约360和30/h的测定速率。最终，这样的发展将导致创建一个现场部署的微量分析仪，并将使法医实验室运输到样本源。
A single-channel chip-based analytical microsystem that allows rapid flow injection measurements of the total content of organic explosive or nerve agent compounds, as well as detailed micellar chromatographic identification of the individual ones, is described. The protocol involves repetitive rapid flow injection (screening) assays to provide a timely warning and alarm-and switching to the separation (fingerprint identification) mode only when harmful compounds are detected. While micellar electro-kinetic chromatography, in the presence of sodium dodecyl sulfate (SDS), is used for separating the neutral nitroaromatic explosive and nerve agent compounds, an operation without SDS leads to high-speed measurements of the "total" explosives or nerve agent content. Switching between the "flow injection" and "separation" modes is accomplished by rapidly exchanging the SDS-free and SDS-containing buffers in the separation channel. Amperometric detection was used for monitoring the separation. Key factors influencing the sample throughput, resolution, and sensitivity have been assessed and optimized. Assays rates of about 360 and 30/h can thus be realized for the "total" screening and "individual" measurements, respectively. Ultimately, such development will lead to the creation of a field-deployable microanalyzer and will enable transporting the forensic laboratory to the sample source.