Gd@C82-(ethylenediamine)8 Nanoparticle: A New High-Efficiency Water -Soluble ROS Scavenger
Gd@C82-(ethylenediamine)8 Nanoparticle: A New High-Efficiency Water -Soluble ROS Scavenger
复制标题
Gd@C-82-(乙二胺)(8)纳米颗粒：一种新型高效水溶性ROS清除剂
DOI：
10.1021/acsami.6b08659
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发表时间：
2016-10-05
影响因子：
9.5
通讯作者：
Wang, Chunru
中科院分区：
文献类型：
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作者：
Li, Jie;Guan, Mirong;Wang, Chunru
维持活性氧（ROS）在生物体内的平衡至关重要，因此，迫切需要一种具有良好生物相容性的有效ROS清除剂。为了制备高效活性氧清除剂，首次将多个乙二胺（EDA）基团键合到金属富勒烯Gd@C-82上，通过简单的固液反应得到水溶性Gd@C-82-（EDA）（8）纳米颗粒（NPs）。具有相对较好的缀合的Gd@C-82-（EDA）（8）NPs具有优异的清除羟基自由基的能力。此外，Gd@C-82-（EDA）（8）NPs在2.5 μ M的低浓度下对H2 O2诱导的人表皮角质形成细胞-成体（HEK-a）细胞损伤表现出显著的细胞保护作用。相比之下，用羟基修饰的Gd@C-82-（OH）（26）NP在40 μ M的高得多的浓度下显示出明显的保护作用。Gd@C-82-（EDA）（8）NPs的这种出色的细胞保护性能主要归因于其极高的细胞摄取和极强的缀合。Gd@C-82-（EDA）（8）具有良好生物相容性的纳米颗粒即使在非常低的浓度下也表现出优异的ROS清除能力，这促进了其在化妆品和生物医学中的广泛应用。
It is important to maintain a reactive oxygen species (ROS) balance in organisms; thus, a valid ROS scavenger with good biocompatibility is urgently required. To prepare a high-efficiency ROS scavenger, multiple ethylenediamine (EDA) groups are bonded for the first time to a metallofullerene Gd@C-82 to obtain water-soluble Gd@C-82-(EDA)(8) nanoparticles (NPs) through a facile solid liquid reaction. Gd@C-82-(EDA)(8) NPs with a relatively better conjugation possess an excellent capability to scavenge hydroxyl radicals. Moreover, Gd@C-82-(EDA)(8) NPs exhibited a remarkable cytoprotective effect against H2O2-induced injuries to human epidermal keratinocytes-adult (HEK-a) cells at a low concentration of 2.5 mu M. In contrast, Gd@C-82-(OH)(26) NPs that modified with hydroxyls show an apparent protective effect at a much higher concentration of 40 mu M. This outstanding cytoprotective performance of Gd@C-82-(EDA)(8) NPs is mainly attributed to their extremely high cellular uptake and comparably strong conjugation. Gd@C-82-(EDA)(8) NPs with good biocompatibility exhibit excellent ROS scavenging capability even at a significantly low concentration, which promotes its versatile applications in cosmetics and biomedicine.