CYTOGENETIC ANALYSIS OF UNFERTILIZED HUMAN OOCYTES
CYTOGENETIC ANALYSIS OF UNFERTILIZED HUMAN OOCYTES
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DOI：
10.1093/oxfordjournals.humrep.a137381
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发表时间：
1991-04-01
影响因子：
6.1
通讯作者：
BAIRD, DT
中科院分区：
文献类型：
--
作者：
ANGELL, RR;LEDGER, W;BAIRD, DT
对180个体外受精后未受精的卵母细胞进行了细胞遗传学研究。 大多数的135个是信息的第二次减数分裂中期，两个是二倍体，和五个有各种不同的异常。 21个卵母细胞异常受精，包括过早凝聚的精子染色体。 这种现象的频率根据刺激方案而变化，那些在体内成熟时间较长的卵母细胞表现出较低的异常受精倾向。 13%的可分析的单倍体中期分裂相是超单倍体，但没有一个含有额外的完整染色体。 额外的成分是一个单一的染色单体（1例），或两个单一的染色单体取代整个染色体（4例）。 这些数据表明，染色单体的出现是由于过早的着丝粒分裂在减数分裂I，这可能是一个主要的机制，而不是整个二价体在减数分裂I不分离，因为通常认为三体形成。
Cytogenetic studies were carried out on 180 oocytes that appeared unfertilized after in-vitro fertilization. The majority of the 135 that were informative had grossly haploid second meiotic metaphases, two were grossly diploid, and five had a variety of different abnormalities. Twenty-one oocytes were abnormally fertilized and included prematurely condensed sperm chromosomes. The frequency of this phenomenon varied according to the stimulation protocol, those oocytes maturing longer in vivo showing less propensity to abnormal fertilizations. Thirteen per cent of the analysable haploid metaphases were hyperhaploid but none contained extra whole chromosomes. The extra components were a single chromatid (one case), or two single chromatids replacing a whole chromosome (four cases). The data suggest that the chromatids arose as a result of premature centromere division at meiosis I, and that this may be a major mechanism for trisomy formation rather than non-disjunction of whole bivalents at meiosis I, as generally believed.