Evidence for the steroid-induced accumulation of tyrosine-aminotransferase messenger RNA in the absence of protein synthesis.
Evidence for the steroid-induced accumulation of tyrosine-aminotransferase messenger RNA in the absence of protein synthesis.
复制标题
在没有蛋白质合成的情况下类固醇诱导酪氨酸转氨酶信使 RNA 积累的证据。
DOI：
10.1073/pnas.60.1.222
复制
发表时间：
1968
影响因子：
11.1
通讯作者：
G. Tomkins
中科院分区：
文献类型：
--
作者：
B. Peterkofsky;G. Tomkins
我们一直在研究糖皮质激素在已建立的组织培养细胞系（HTC细胞）中诱导TAT 1的机制。2该系统使我们能够使用完整的动物进行技术上困难或不可能的实验。虽然用类固醇处理这些组织培养细胞后未观察到RNA合成的总体变化，但根据抑制剂研究得出结论，酶特异性mRNA的合成和积累是诱导所必需的。4本实验旨在确定类固醇激素是否可以在蛋白质合成不存在的情况下刺激达特mRNA的出现。可以提出几种诱导机制，其中mRNA积累需要蛋白质合成。例如，激素的初始作用可能是增加达特mRNA本身的翻译速率，这可能会导致其积累;或者达特诱导可能是其他酶的先前诱导的结果，然后产生达特的“真正诱导物”。然而，本文中描述的实验表明，蛋白质合成对于用类固醇处理HTC细胞产生的TAT特异性RNA的积累是不需要的。因此，我们得出结论，激素直接作用于增加mRNA合成或减少其降解，导致其在细胞中的浓度增加。
We have been studying the mechanism of TAT1 induction by glucocorticoid hormones in an established line of tissue culture cells (HTC cells).2 This system allows us to perform experiments which would be technically difficult or impossible using intact animals. Although no gross changes in RNA synthesis were observed after treating these tissue culture cells with steroid, it was concluded on the basis of inhibitor studies that the synthesis and accumulation of enzymespecific mRNA is required for induction.4 The present experiments were designed to determine whether steroid hormone could stimulate the appearance of TAT mRNA in the absence of protein synthesis. Several mechanisms of induction could be proposed in which protein synthesis would be required for mRNA accumulation. For example, the initial effect of the hormone could be to increase the rate of translation of the TAT mRNA itself which could secondarily lead to its accumulation; or TAT induction might be the consequence of the prior induction of other enzymes which then produce the "true inducer" of TAT. The experiments described in this paper, however, show that protein synthesis is not needed for the accumulation of TATspecific RNA resulting from treatment of HTC cells with steroid. We therefore conclude that the hormone acts directly either to increase mRNA synthesis or to decrease its degradation resulting in an increase in its concentration in the cell.