Herpes simplex virus glycoproteins associated with different morphological entities projecting from the virion envelope.
Herpes simplex virus glycoproteins associated with different morphological entities projecting from the virion envelope.
复制标题
单纯疱疹病毒糖蛋白与从病毒体包膜突出的不同形态实体相关。
DOI：
10.1099/0022-1317-68-3-715
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Spear,PG
中科院分区：
文献类型：
--
作者：
Stannard,LM;Fuller,AO;Spear,PG
通过负染色制剂的电子显微镜检查，在单纯疱疹病毒粒子的表面上检测到不同种类、不同大小和外观的尖峰。使用与胶体金偶联的单克隆抗体可以鉴定病毒体包膜突出的不同结构中存在的病毒糖蛋白。对指定为 gB 的糖蛋白具有特异性的抗体与最突出的尖峰结合，这些尖峰长约 14 nm，从侧面看有一个扁平的 T 形顶部。 gC 特异性抗体与结构结合，在某些情况下，这些结构似乎从包膜表面延伸 24 nm，而且太薄而无法解析。 gD 特异性抗体与从包膜表面延伸 8 至 10 nm 的结构结合。 gB 尖峰总是聚集在一起，通常位于包膜的突出部分，从小球状膨胀到长尾状突出不等。 gC 成分随机分布且间隔较宽，gD 成分不规则地聚集，其模式与 gB 尖峰的模式不同。因此，这三种糖蛋白在包膜中形成大小、形态和分布不同的结构。
Spikes of different kinds, distinct in size and appearance were detected on the surfaces of herpes simplex virions by electron microscopy of negatively stained preparations. Use of monoclonal antibodies coupled to colloidal gold permitted identification of viral glycoproteins present in different structures projecting from the virion envelope. Antibodies specific for the glycoprotein designated gB bound to the most prominent spikes, which were about 14 nm long and, in side view, had a flattened T-shaped top. Antibodies specific for gC bound to structures that, in some instances, appeared to extend as much as 24 nm from the surface of the envelope and were too thin to resolve. Antibodies specific for gD bound to structures that extended as much as 8 to 10 nm from the surface of the envelope. The gB spikes were invariably clustered, usually in protrusions of the envelope varying from small bulbous distentions to long tail-like projections. The gC components were randomly distributed and widely spaced and the gD components were irregularly clustered in patterns distinct from those of the gB spikes. These three glycoproteins therefore form structures that are different in size, morphology and distribution in the envelope.