Photographic quantitation of DNA in gel electrophoresis.
Photographic quantitation of DNA in gel electrophoresis.
复制标题
凝胶电泳中 DNA 的照相定量。
DOI：
10.1016/0003-2697(77)90008-2
复制
发表时间：
1977
影响因子：
2.9
通讯作者：
B. Leblanc
中科院分区：
文献类型：
--
作者：
A. Prunell;F. Strauss;B. Leblanc
本文描述了一种用溴化乙锭染色后通过凝胶电泳定量DNA条带的摄影方法。确定了薄膜变暗与照射到薄膜上的光强度之间的关系。在底片的密度描记中测量的密度被转换成荧光强度。发现荧光与DNA的量成线性比例。由于凝胶超载，不均匀的电泳迁移和紫外光照射，溴化乙锭的光分解的偏差进行了研究。
A photographic procedure to quantitate the DNA in bands, obtained by gel electrophoresis after staining with ethidium bromide, is described. The relationship between the film darkening and the intensity of the light hitting the film was determined. The densities, measured in densitometric tracings of the negatives, were converted into fluorescence intensities. The fluorescence was found to be linearly proportional to the amount of DNA. Deviations due to gel overloading, to nonuniform electrophoretic migration and uv illumination, and to photodecomposition of ethidium bromide were investigated.