Fatty acid synthase expression in melanoma
Fatty acid synthase expression in melanoma
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DOI：
10.1034/j.1600-0560.2003.300104.x
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发表时间：
2003-01-01
影响因子：
1.7
通讯作者：
Calvieri, S
中科院分区：
文献类型：
--
作者：
Innocenzi, D;Alò, PL;Calvieri, S
背景：脂肪酸合成酶(Fas)是合成脂肪酸的关键酶，在人体某些正常组织中弱表达。近年来，Fas在许多非肿瘤性高增生性病变和侵袭性癌(包括结肠癌、乳腺癌和卵巢癌)中均有过表达。方法：采用免疫组织化学方法，对77例原发黑色素瘤和30例淋巴结和皮肤转移的黑色素瘤进行免疫组织化学分析，以探讨Fas在黑色素瘤中的预后意义。30例(真皮和交界性各15例)作为对照。结果：34例黑色素瘤Fas免疫组织化学染色呈强阳性，其余43例呈弱表达或阴性。所有皮肤和淋巴结转移均为强阳性。所有转移的患者在随访期内均死亡。对照标本染色较弱。这些患者无一例复发。统计学分析显示Fas表达与Breslow厚度显著相关(p=0.012)。Fas免疫染色强度对预后也有预测作用(p=0.049)。结论：Fas是判断黑色素瘤预后的可靠指标。Fas的预测强度随着Breslow厚度的增加而增加。对人类黑色素瘤中Fas的观察可能会对患者进行分层，以便进行更严格的随访，并建议不同的治疗方法。
Background: Fatty acid synthase (FAS), the key enzyme responsible for the synthesis of fatty acids, is weakly expressed in some normal human tissues. Recently, FAS has been demonstrated to be overexpressed in many non-neoplastic highly proliferative lesions and in aggressive carcinomas with poor outcome, including colon, breast and ovary carcinomas.Methods: In order to evaluate the prognostic significance of FAS in human melanoma, we analysed by means of immunohistochemistry, using a monoclonal anti-FAS antibody, 77 primary melanomas and 30 nodal and cutaneous metastasis. Thirty nevi (15 dermal and 15 junctional nevi) were used as controls. All patients were followed-up for 5 years.Results: Thirty-four melanomas expressed strong FAS immunostaining; the remaining 43 cases showed weak expression or were negative. All cutaneous and nodal metastasis were strongly positive. All patients with metastases deceased during the follow up period. Control specimens expressed weak staining. None of these patients developed recurrence. Statistical analysis revealed significant association of FAS expression with Breslow thickness (p=0.012). The intensity of FAS immunostaining was also predictive of prognosis (p=0.049).Conclusions: FAS is a reliable prognostic marker in human melanomas. FAS predictive strength is increased when associated with Breslow thickness. The observation of FAS in human melanomas may stratify patients for stricter follow-ups and suggest different therapeutic approaches.