Polaris, a protein involved in left-right axis patterning, localizes to basal bodies and cilia
Polaris, a protein involved in left-right axis patterning, localizes to basal bodies and cilia
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DOI：
10.1091/mbc.12.3.589
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发表时间：
2001-03-01
影响因子：
3.3
通讯作者：
Yoder, BK
中科院分区：
文献类型：
--
作者：
Taulman, PD;Haycraft, CJ;Yoder, BK
Tg 737的突变导致广泛的表型，包括随机左右轴特化、多囊肾病、肝脏和胰腺缺陷、脑积水和骨骼模式异常。为了进一步评估Tg 737的生物学功能及其在突变病理学中的作用，我们鉴定了表达Tg 737的细胞群，并确定了其蛋白产物Polaris的亚细胞定位。TG 737表达与具有运动或不运动纤毛的细胞和精子有关。同样，北极星集中在顶膜下面的区域的基体和纤毛或鞭毛轴丝内。这些数据表明，北极星的功能在ciliogenic途径或纤毛的维护，纤毛的损失支持的室管膜细胞层在Tg 7370（orpk）的脑室和缺乏节点纤毛Tg 737（Δ 2 -3 β Gal）突变体的作用。
Mutations in Tg737 cause a wide spectrum of phenotypes, including random left-right axis specification, polycystic kidney disease, liver and pancreatic defects, hydrocephalus, and skeletal patterning abnormalities. To further assess the biological function of Tg737 and its role in the mutant pathology, we identified the cell population expressing Tg737 and determined the subcellular localization of its protein product called Polaris. Tg737 expression is associated with cells possessing either motile or immotile cilia and sperm. Similarly, Polaris concentrated just below the apical membrane in the region of the basal bodies and within the cilia or flagellar axoneme. The data suggest that Polaris functions in a ciliogenic pathway or in cilia maintenance, a role supported by the loss of cilia on the ependymal cell layer in ventricles of Tg7370(orpk) brains and by the lack of node cilia in Tg737(Delta2-3 beta Gal) mutants.