Probing embryonic tissue mechanics with laser hole drilling
Probing embryonic tissue mechanics with laser hole drilling
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DOI：
10.1088/1478-3975/6/3/036004
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发表时间：
2009-09-01
期刊：
影响因子：
2
通讯作者：
Hutson, M. Shane
中科院分区：
文献类型：
--
作者：
Ma, Xiaoyan;Lynch, Holley E.;Hutson, M. Shane
我们使用激光打孔来评估胚胎上皮在体内发育过程中的力学和亚细胞分辨率。我们消融一个亚细胞圆柱形孔，穿过上皮，并跟踪相邻细胞的后续反冲（ms时间尺度）。我们研究果蝇的背侧闭合，重点是浆膜细胞的顶端收缩。该上皮的力学行为介于连续片材和2D多孔泡沫（拉伸界面的网络）之间福尔斯。张应力由细胞-细胞界面和细胞顶端肌动蛋白网络承载。我们的结果表明，应力稍微集中沿着接口（1.6倍），但仅在早期关闭。此外，关闭的特点是减少反冲幂律指数，这意味着过渡到一个更坚实的组织。我们使用的网站和阶段的依赖性的反冲动力学来约束如何关闭过程中的细胞力学变化。我们应用这些结果来测试现存的计算模型。
We use laser hole drilling to assess the mechanics of an embryonic epithelium during development-in vivo and with subcellular resolution. We ablate a subcellular cylindrical hole clean through the epithelium and track the subsequent recoil of adjacent cells (on ms time scales). We investigate dorsal closure in the fruit fly with emphasis on apical constriction of amnioserosa cells. The mechanical behavior of this epithelium falls between that of a continuous sheet and a 2D cellular foam (a network of tensile interfaces). Tensile stress is carried both by cell-cell interfaces and by the cells' apical actin networks. Our results show that stress is slightly concentrated along interfaces (1.6-fold), but only in early closure. Furthermore, closure is marked by a decrease in the recoil power-law exponent, implying a transition to a more solid-like tissue. We use the site and stage dependence of the recoil kinetics to constrain how the cellular mechanics change during closure. We apply these results to test extant computational models.