Inhibition of Potassium Uptake and Regulation of Membrane-Associated Mg2+-ATPase Activity of Pea Roots by Aluminium
Inhibition of Potassium Uptake and Regulation of Membrane-Associated Mg2+-ATPase Activity of Pea Roots by Aluminium
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铝对豌豆根部钾吸收的抑制及膜相关Mg2-ATP酶活性的调节
DOI：
10.1080/00380768.1986.10557495
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发表时间：
1986
影响因子：
2
通讯作者：
T. Yamaya
中科院分区：
文献类型：
--
作者：
H. Matsumoto;T. Yamaya
AIC13处理显著降低了豌豆对K+的吸收。Ca~(2+)的共同作用可减轻这种下降。从豌豆根中分离得到的ATPase结合膜被认为是富含结合ATPase性质的质膜。该酶的活性高度依赖于镁离子的存在，而不依赖于钙离子的存在，在中性pH条件下酶活性最高。二乙基己烯甾醇、N，N‘-二环己基碳二亚胺对该酶有抑制作用。和钒酸盐。以三磷酸腺苷为底物时，对三磷酸腺苷的要求比较特殊。膜相关的ATPase被加入到测定介质中的AIC13竞争性地抑制。Al在不同pH时的抑制率与无AI时的抑制率呈平行关系。不同的化学物质被用来减轻AI对膜相关ATPase的抑制。三聚磷酸盐、柠檬酸、葡萄糖6-磷酸、焦磷酸盐、谷氨酸和苹果酸的修复.
The uptake of K+ by pea decreased markedly by the treatment with AICl3. The decrease was alleviated by the co-treatment with Ca2+. The membrane associated with ATPase which was isolated from pea roots, was considered to be the plasma membrane enriched by the natures of associated ATPase. The activity was highly dependent on the presence of Mg2+, but not Ca2+ and the optimum activity was observed at neutral pH. The activity was inhibited by diethylstilbesterol, N,N’ -dicyclohexyl carbodiimide. and vanadate. Rather specific requirement of ATP was observed when ATP acted as a substrate. The membrane-associated ATPase was competitively inhibited by AICl3 added to the assay medium with respect to ATP. The rate of inhibition by Al at various pHs was parallel with that of the activity in the absence of AI. Various chemicals were tested for the alleviation of the inhibition of membrane-associated ATPase by AI. Tripolyphosphate, citric acid, glucose 6-phosphate, pyrophosphate, glutamic acid, and malic acid restore...