FUNCTIONAL DIFFERENCES BETWEEN AFFERENT-FIBERS IN THE HYPOGASTRIC AND PELVIC NERVES INNERVATING FEMALE REPRODUCTIVE-ORGANS IN THE RAT
FUNCTIONAL DIFFERENCES BETWEEN AFFERENT-FIBERS IN THE HYPOGASTRIC AND PELVIC NERVES INNERVATING FEMALE REPRODUCTIVE-ORGANS IN THE RAT
复制标题
DOI：
10.1152/jn.1993.69.2.533
复制
发表时间：
1993-02-01
影响因子：
2.5
通讯作者：
SATO, Y
中科院分区：
文献类型：
--
作者：
BERKLEY, KJ;ROBBINS, A;SATO, Y
1.子宫、宫颈和阴道由腹下神经和盆神经的传入纤维支配。四项研究比较了成年未交配大鼠两组纤维的神经支配范围和对外周刺激的敏感性.通过将不同的荧光染料注射到生殖道、下泌尿道和下消化道的不同部位，并检查背根神经节中的逆行标记神经元，从解剖学上研究了神经支配领域。在麻醉大鼠中，通过电刺激生殖束不同部位，对两种神经分支诱发的电位求和，进行了电生理学研究.在这两项研究中，生殖道的感觉神经支配从骨盆转移到腹下神经（即，分别从L 6-S1到T13-L3背根神经节转移进入脊髓），同时染料或刺激电极从阴道入口转移到子宫角，来自两个神经的纤维密集地支配子宫颈区域（即，通过两组神经节进入脊髓）。解剖学结果提示，一条神经的纤维支配的区域也可能由另一条神经的一小部分正常静息纤维支配.反应灵敏度进行了电生理学研究，同时记录多单位活动的分支腹下神经和盆神经的两种方式。首先，在完整的麻醉大鼠中，在生殖道的机械刺激（阴道和子宫扩张，探测子宫颈）期间记录活动。第二，在子宫和阴道的体外器官制备中，在用缓激肽、5-羟色胺、NaCN、CO2和KCl通过子宫动脉进行化学刺激期间记录活性。盆腔神经纤维对子宫和宫颈机械刺激的敏感性明显高于腹下神经纤维。同样，盆腔神经纤维比腹下神经纤维更可能对所有化学刺激（KCl除外）做出反应或反应更剧烈。这些结果提供了强有力的证据表明，传入纤维的盆神经和腹下神经的未生育的成年大鼠subsides不同的功能，在生殖和感觉。盆神经纤维似乎与交配和受孕相关的感觉和行为过程密切相关，而腹下神经纤维似乎与妊娠和伤害感受密切相关，两种神经中的纤维在分娩期间都发挥作用。
1. The uterus, cervix, and vaginal canal are innervated by afferent fibers in the hypogastric and pelvic nerves. Four studies compared the innervation territory and sensitivity to peripheral stimuli of the two sets of fibers in adult virgin rats.2. Innervation territory was studied anatomically by injecting different fluorescent dyes into different parts of the reproductive, lower urinary, and lower digestive tracts and examining retrogradely labeled neurons in dorsal root ganglia. It was also studied electrophysiologically in anesthetized rats by summing potentials evoked in branches of the two nerves by electrical stimulation of different parts of the reproductive tract.3. In both studies sensory innervation of the reproductive tract shifted from the pelvic to the hypogastric nerve (i.e., shifted entry into the spinal cord from the L6-S1 to the T13-L3 dorsal root ganglia, respectively) as the dye or stimulating electrode shifted from the vaginal entrance to the uterine horns, with fibers from both nerves densely innervating the cervix region (i.e., entering the spinal cord through both sets of ganglia). The anatomic results suggested that the regions innervated by fibers in one nerve might also be innervated by a small component of normally quiescent fibers in the other nerve.4. Response sensitivity was studied electrophysiologically by simultaneously recording multiunit activity in branches of the hypogastric and pelvic nerves in two ways. First, in intact, anesthetized rats, activity was recorded during mechanical stimulation of the reproductive tract (distension of the vagina and uterus, probing the cervix). Second, in an in vitro organ preparation of the uterus and vagina, activity was recorded during chemical stimulation through the uterine artery with bradykinin, serotonin, NaCN, CO2, and KCl.5. Pelvic nerve fibers were markedly more sensitive than hypogastric nerve fibers to uterine and cervical mechanostimulation. Similarly, pelvic nerve fibers were more likely to respond or responded more vigorously than hypogastric nerve fibers to all chemical stimuli (except KCl).6. These results provide strong evidence that afferent fibers in the pelvic and hypogastric nerves of nulliparous adult rats subserve different functions in reproduction and sensation. Pelvic nerve fibers seem closely tied to sensory and behavioral processes associated with mating and conception, whereas hypogastric fibers seem closely tied to pregnancy and nociception, with fibers in both nerves serving functions during parturition.