Adaptation in the motility response to cAMP in Dictyostelium discoideum.
Adaptation in the motility response to cAMP in Dictyostelium discoideum.
复制标题
盘基网柄菌对 cAMP 运动反应的适应。
DOI：
10.1002/cm.970090103
复制
发表时间：
1988
影响因子：
--
通讯作者：
Soll,DR
中科院分区：
文献类型：
--
作者：
Varnum-Finney,B;Schroeder,NA;Soll,DR
当用恒定浓度的cAMP（大于10− 8 M）处理盘状网柄藻的阿米巴时，平均运动速率被抑制，在大约10− 6 M时抑制最大。研究表明，在灌注室中，将cAMP浓度从0 M改变到10 - 8至10 - 6 M范围内，会立即抑制运动。在从0 M转换到10− 8或10− 7 M后，细胞运动的速率保持较低，然后反弹到更高的水平，表现出标准的适应反应。从0 M到10− 6 M（导致最大抑制的浓度）转换后，没有显示出适应性。它表明，抑制的水平和适应期的程度取决于cAMP的浓度后，移动，和次最大抑制是加性的。这种运动性反应的适应特征与中继系统和假定cAMP受体磷酸化的适应特征非常相似。
When developing amebae ofDictyostelium discoideumare treated with constant concentrations of cAMP above 10−8M, the average rate of motility is depressed, with maximum inhibition at roughly 10−6M. It is demonstrated that shifting the concentration of cAMP from 0 M to concentrations ranging from 10−8to 10−6M in a perfusion chamber results in the immediate inhibition of motility. After shifting from 0 M to 10−8or 10−7M, the rate of cell motility remains low, then rebounds to a higher level, exhibiting a standard adaptation response. No adaptation is exhibited after a shift from 0 M to 10−6M, a concentration resulting in maximum inhibition. It is demonstrated that the level of inhibition and the extent of the adaptation period are dependent upon the concentration of cAMP after the shift, and that submaximal inhibition is additive. The characteristics of adaptation in this motility response are very similar to the characteristics of adaptation for the relay system and phosphorylation of the putative cAMP receptor.