Metabolic Engineering of Bacillus licheniformis for Production of Acetoin
Metabolic Engineering of Bacillus licheniformis for Production of Acetoin
复制标题
地衣芽孢杆菌生产乙偶姻的代谢工程
DOI：
10.3389/fbioe.2020.00125
复制
发表时间：
2020-02-21
影响因子：
5.7
通讯作者：
Ma, Cuiqing
中科院分区：
文献类型：
--
作者：
Lu, Chuanjuan;Ge, Yongsheng;Ma, Cuiqing
乙托因是一种潜在的平台化合物，可用于多种化学品。地衣芽孢杆菌（Bacillus licheniformis MW3）是一种嗜热且通常被认为是安全的（GRAS）微生物，可以高浓度、高产、高产地生产2,3-丁二醇。在本研究中，地衣芽胞杆菌MW3被代谢工程用于乙酰素的生产。在删除编码budC和gdh基因的两个2,3-丁二醇脱氢酶后，构建了地衣芽孢杆菌MW3 （ΔbudCΔgdh）工程菌株。对地衣芽孢杆菌MW3 （ΔbudCΔgdh）进行补料分批发酵，从156 g/L葡萄糖中获得64.2 g/L的乙酰素，27 h产率为2.378 g/[L h]，产率为0.412 g/g，表明地衣芽孢杆菌MW3 （ΔbudCΔgdh）可能是一种很有前途的乙酰素产源。
Acetoin is a potential platform compound for a variety of chemicals. Bacillus licheniformis MW3, a thermophilic and generally regarded as safe (GRAS) microorganism, can produce 2,3-butanediol with a high concentration, yield, and productivity. In this study, B. licheniformis MW3 was metabolic engineered for acetoin production. After deleting two 2,3-butanediol dehydrogenases encoding genes budC and gdh, an engineered strain B. licheniformis MW3 (ΔbudCΔgdh) was constructed. Using fed-batch fermentation of B. licheniformis MW3 (ΔbudCΔgdh), 64.2 g/L acetoin was produced at a productivity of 2.378 g/[L h] and a yield of 0.412 g/g from 156 g/L glucose in 27 h. The fermentation process exhibited rather high productivity and yield of acetoin, indicating that B. licheniformis MW3 (ΔbudCΔgdh) might be a promising acetoin producer.