Role of Salmonella enterotoxin in overall virulence of the organism
Role of Salmonella enterotoxin in overall virulence of the organism
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DOI：
10.1006/mpat.1999.0294
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发表时间：
1999-09-01
影响因子：
3.8
通讯作者：
Peterson, JW
中科院分区：
文献类型：
--
作者：
Chopra, AK;Huang, JH;Peterson, JW
在这项研究中，沙门氏菌的肠毒素基因（ESTO）的突变标记交换诱变，并评价了该生物体的总体毒力。沙门氏菌标志物交换突变体诱发明显减少液体分泌小鼠肠袢相比，野生型S。鼠伤寒。沙门氏菌突变体的侵袭性作为野生型细菌的HeLa细胞，然而，它们的能力，造成破坏的肠粘膜受损，与野生型细菌相比，电子显微镜。小鼠经口攻击后，沙门氏菌突变体的LD 50大于野生型细菌。液体分泌的潜力，以及这些突变体的LD 50的减少恢复时，突变的p53基因被替换的天然p53基因序列。这些突变对这些细菌在基本培养基或完全培养基中的需氧生长没有影响;厌氧生长也不受影响。通过这些研究，我们证明了完整的p53基因的存在对沙门氏菌的总体毒力有显著的贡献。鼠模型中的鼠伤寒沙门氏菌。(C)北京：科学出版社.
In this study, the Salmonella enterotoxin gene (stn) was mutated by marker exchange mutagenesis, and the overall virulence of the organism was evaluated. Salmonella marker exchange mutants evoked significantly less fluid secretion in mouse intestinal loops compared to that seen with wildtype S. typhimurium. Salmonella mutants were as invasive as wild-type bacteria for HeLa cells; however, their capacity to cause destruction of the intestinal mucosa was impaired, when compared with wild-type bacteria by electron microscopy. Upon oral challenge of mice, the LD50 of the Salmonella mutants was greater than that for the wild-type bacteria. The fluid secretory potential, as well as a reduction in the LD50 of these mutants was restored when the mutated stn gene was replaced by the native stn gene sequence. These mutations had no effect on the aerobic growth of these bacteria in minimal or complete medium; anaerobic growth was also not affected. With these studies, we demonstrated that the presence of an intact stn gene contributed significantly to the overall virulence of S. typhimurium in a murine model. (C) 1999 Academic Press.