Binding of pulmonary surfactant proteins to carbon nanotubes;: potential for damage to lung immune defense mechanisms
Binding of pulmonary surfactant proteins to carbon nanotubes;: potential for damage to lung immune defense mechanisms
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DOI：
10.1016/j.carbon.2006.10.011
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发表时间：
2007-03-01
期刊：
影响因子：
10.9
通讯作者：
Sim, Robert B.
中科院分区：
文献类型：
--
作者：
Salvador-Morales, Carolina;Townsend, Paul;Sim, Robert B.
碳纳米管的潜在肺毒性是一个受到广泛关注的研究领域。表面活性蛋白 A 和 D（SP-A 和 SP-D）是由肺部气道上皮细胞分泌的集合素蛋白。它们在抵抗肺部感染的一线防御中发挥着重要作用。本研究的目的是研究碳纳米管与肺表面活性剂中所含蛋白质之间的相互作用。通过使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SIDS-PAGE）、蛋白质印迹（一种基于碳纳米管-琼脂糖基质的亲和层析新技术[1]）和电子显微镜数据，表明SP-A和SP-D选择性地与碳纳米管结合。结合是Ca2+离子依赖性的，并且在不同批次的纳米管之间是可变的。因此，它可能是由纳米管的表面杂质或化学修饰介导的。长期接触碳纳米管可能会导致 SP-D 和 SP-A 的隔离。基因敲除小鼠缺乏这些蛋白质会导致肺部感染和肺气肿的易感性。 (c) 2006 Elsevier Ltd. 保留所有权利。
Potential pulmonary toxicity of carbon nanotubes is a research area that has received considerable attention. Surfactant proteins A and D (SP-A and SP-D) are collectin proteins that are secreted by airway epithelial cells in the lung. They play an important role in first-line defense against infection within the lung. The aim of this study was to investigate the interaction between carbon nanotubes and proteins contained in lung surfactant. By using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SIDS-PAGE), Western Blotting, a novel technique of affinity chromatography based on carbon nanotube-Sepharose matrix [1] and electron microscopy data it was shown that SP-A and SP-D selectively bind to carbon nanotubes. The binding was Ca2+-ion dependent, and was variable between batches of nanotubes. It was therefore likely to be mediated by surface impurities or chemical modifications of the nanotubes. Chronic level exposure to carbon nanotubes may result in sequestration of SP-D and SP-A. Absence of these proteins in knockout mice leads to susceptibility to lung infection and emphysema. (c) 2006 Elsevier Ltd. All rights reserved.