ENHANCED TRANSCRIPTION OF C-MYC IN BURSAL LYMPHOMA-CELLS REQUIRES CONTINUOUS PROTEIN-SYNTHESIS
ENHANCED TRANSCRIPTION OF C-MYC IN BURSAL LYMPHOMA-CELLS REQUIRES CONTINUOUS PROTEIN-SYNTHESIS
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DOI：
10.1126/science.2999973
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
GROUDINE, M
中科院分区：
文献类型：
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作者：
LINIAL, M;GUNDERSON, N;GROUDINE, M
在一些c-mycas转录受禽白血病病毒（ALV）长末端重复序列（LTR）调控的法氏囊淋巴瘤细胞系中，蛋白质合成抑制降低了c-mycas以及其他LTR驱动的病毒基因的转录活性。这种转录的减少与c-myc染色质结构的变化有关，通过脱氧核糖核酸酶I （DNase I）超敏测量，转录从LTR转移到正常的c-myc启动子。相比之下，环己亚胺对感染的鸡胚成纤维细胞LTR驱动基因的转录几乎没有影响。这些结果表明，一种不稳定的细胞类型特异性蛋白可能与逆转录病毒LTR相互作用，并调节LTR控制基因的转录。此外，结果表明，环己亚胺处理正常细胞诱导的细胞内c-mycRNA浓度的增加是该信息稳定的结果。
In several bursal lymphoma cell lines in which c-myctranscription is regulated by avian leukosis virus (ALV) long terminal repeat (LTR) sequences, protein synthesis inhibition decreases the transcriptional activity of c-mycas well as other LTR driven viral genes. This decrease in transcription is associated with a change in the chromatin structure of c-myc, as measured by deoxyribonuclease I (DNase I) hypersensitivity, and a shift of transcription from the LTR to the normal c-mycpromoter. In contrast, cycloheximide had little or no effect on the transcription of LTR driven genes in infected chicken embryo fibroblasts treated with the drug. These results suggest that a labile, cell type-specific protein may interact with the retroviral LTR and regulate transcription of genes under LTR control. Further, the results demonstrate that the increase in intracellular concentration of c-mycRNA induced by cycloheximide treatment of normal cells is the result of stabilization of this message.