Muscle satellite cells: A validated technique for light microscopic identification and a quantitative study of changes in their population following denervation
Muscle satellite cells: A validated technique for light microscopic identification and a quantitative study of changes in their population following denervation
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肌肉卫星细胞：一种经过验证的光学显微镜识别技术以及去神经支配后其群体变化的定量研究
DOI：
10.1002/ar.1091780206
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
M. Ontell
中科院分区：
文献类型：
--
作者：
M. Ontell
将碱性品红、亚甲蓝和天青II应用于包埋在环氧树脂中的0.5 μ切片的大鼠横纹肌的染色技术，充分区分了在肌束中发现的五种核类型，以保证其用作在光学显微镜下鉴定卫星细胞的有效方法。该技术的有效性进行了评估，通过研究系列薄和厚的部分失神经大鼠后肢肌肉。在电子显微镜下检查光显微镜“分型”的细胞核，以确定它们所在的细胞类型。然后应用这种染色技术来确定去神经支配对胫骨前肌和趾长伸肌卫星细胞群的影响。在200 gm大鼠的对照肌肉中，卫星细胞占总核群的2%。失神经后2周和3周分别占6%和12%。在800 gm大鼠中，卫星细胞占正常肌肉中发现的总细胞核的0.7%。去神经支配使百分比增加到3%（2周）和5%（3周）。很少观察到成对的卫星细胞;然而，在0.5 μ切片中，发现大量卫星细胞来自同一纤维的肌核，小于0.7 μ。提出了一个模型来解释卫星细胞的增加以下去神经支配。
A staining technique, in which basic fuchsin, methylene blue, and Azure II is applied to 0.5 μ sections of striated rat muscle embedded in epoxy resin, has given sufficient differentiation of five nuclear types found in the muscle fascicle to warrant its use as a valid method for identifying satellite cells with the light microscope. The validity of this technique was assessed by studying serial thin and thick sections of denervated rat hind limb muscles. Nuclei “typed” light microscopically were examined under the electron microscope to determine the type of cell in which they were located. This stain technique was then applied to determine the effect of denervation on the satellite cell population of the tibialis anterior and extensor digitorum longus. In control muscle of 200 gm rats, satellite cells represented 2% of the total nuclear population. Two and three weeks after denervation they accounted for 6% and 12%, respectively. In 800 gm rats, satellite cells accounted for 0.7% of the total nuclei found in the normal muscle. Denervation increased the percentage to 3% (at 2 weeks) and 5% (at 3 weeks). Paired satellite cells were infrequently observed; however, in 0.5 μ sections a significant number of satellite cells were found to be less than 0.7 μ from a myonucleus of the same fiber. A model is proposed to explain the increase in satellite cells following denervation.