Comparison of cell growth in T-flasks, in micro hollow fiber bioreactors, and in an industrial scale hollow fiber bioreactor system
Comparison of cell growth in T-flasks, in micro hollow fiber bioreactors, and in an industrial scale hollow fiber bioreactor system
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DOI：
10.1023/a:1008167713696
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发表时间：
2000-10-01
期刊：
影响因子：
2.2
通讯作者：
Poeschl, DM
中科院分区：
文献类型：
--
作者：
Gramer, MJ;Poeschl, DM
本文将新型微型中空纤维生物反应器中的细胞生长情况与t型烧瓶和大型工业中空纤维生物反应器AcuSyst-Maximizer(R)中的细胞生长情况进行了比较。在t型瓶中，一个小鼠杂交瘤在三种不同培养基中的生长速度与另外三个小鼠杂交瘤在一种培养基中的生长速度差异相对较小。然而，在这些相同的条件下，微生物反应器中细胞的生长速率存在实质性差异。这些差异与Maximizer中相应的初始细胞扩增率密切相关。Maximizer中稳态抗体产率的定量预测更有问题。然而，在微生物反应器中导致更快的初始细胞生长和更高的活细胞密度的条件与Maximizer中细胞系的更好性能相关。这些结果表明，在确定大规模中空纤维生物反应器系统中细胞生长的最佳条件时，微型生物反应器比t型烧瓶更有用。
In this article, cell growth in a novel micro hollow fiber bioreactor was compared to that in a T-flask and the AcuSyst-Maximizer(R), a large scale industrial hollow fiber bioreactor system. In T-flasks, there was relatively little difference in the growth rates of one murine hybridoma cultured in three different media and for three other murine hybridomas cultured in one medium. However, substantial differences were seen in the growth rates of cells in the micro bioreactor under these same conditions. These difference correlated well with the corresponding rates of initial cell expansion in the Maximizer. Quantitative prediction of the steady-state antibody production rate in the Maximizer was more problematic. However, conditions which lead to faster initial cell growth and higher viable cell densities in the micro bioreactor correlated with better performance of a cell line in the Maximizer. These results demonstrate that the micro bioreactor is more useful than a T-flask for determining optimal conditions for cell growth in a large scale hollow fiber bioreactor system.