Excitatory and inhibitory switches for courtship in the brain of Drosophila melanogaster
Excitatory and inhibitory switches for courtship in the brain of Drosophila melanogaster
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DOI：
10.1016/j.cub.2004.03.037
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发表时间：
2004-04-06
期刊：
影响因子：
9.2
通讯作者：
Greenspan, RJ
中科院分区：
文献类型：
--
作者：
Broughton, SJ;Kitamoto, T;Greenspan, RJ
背景资料：求偶是果蝇中研究得最多的行为，其解剖学基础的研究主要集中在大脑中两性异形部位的功能鉴定上。少得多的是已知的更广泛的，nondimorphic，但仍然是必不可少的，神经元subserving男性求爱behavior.Results：网站在中枢神经系统介导的启动和早期步骤的男性求爱果蝇确定通过分析的行为，马赛克苍蝇表达转基因设计抑制神经传递或增强神经元的兴奋性。抑制神经传递是通过显性作用，温度敏感的发动蛋白突变shibire（ts1），而增强的神经元兴奋性是通过一种新的，显性作用，截短的eag钾通道。通过使用一种新的、基于里程碑的程序来比对各种嵌合体菌株之间的不同表达模式，对表达转基因的菌株中的求偶性能和受影响的大脑部位进行了比较，确定了后外侧原大脑中的一簇细胞，它们对求偶的启动产生相互作用，当它们失活时抑制求偶，当它们过度激活时增强求偶，这表明细胞通常起着兴奋、触发的作用。一个单独的组附近的细胞，稍前在侧前脑，发现抑制求偶时，其活动增强，表示在courtship.Conclusions的抑制作用：一个集群的细胞，一些兴奋和一些抑制，在侧前脑调节求偶开始在果蝇。这些细胞可能是触发雄性求爱的多种感觉输入的整合中心。
Background: Courtship is the best-studied behavior in Drosophila melanogaster, and work on its anatomical basis has concentrated mainly on the functional identification of sexually dimorphic sites in the brain. Much less is known of the more expansive, nondimorphic, but nonetheless essential, neural elements subserving male courtship behavior.Results: Sites in the CNS mediating initiation and early steps of male courtship in Drosophila melanogaster were identified by analyzing the behavior of mosaic flies expressing transgenes designed either to suppress neurotransmission or enhance neuronal excitability. Suppression of neurotransmission was accomplished by means of the dominantly acting, temperature-sensitive dynamin mutation shibire(ts1), whereas enhanced neuronal excitability was produced by means of a novel, dominantly acting, truncated eag potassium channel. By using a new, landmark-based procedure for aligning diverse expression patterns among the various mosaic strains, a comparison of courtship performance and affected brain sites in strains expressing the transgenes identified a cluster of cells in the posterior lateral proto-cerebrum that exerts reciprocal effects on the initiation of courtship, suppressing it when they are inactivated and enhancing it when they are hyperactivated, indicative of cells that normally play an excitatory, triggering role. A separate group of nearby cells, slightly more anterior in the lateral protocerebrum, was found to inhibit courtship when its activity is enhanced, indicative of an inhibitory role in courtship.Conclusions: A cluster of cells, some excitatory and some inhibitory, in the lateral protocerebrum regulates courtship initiation in Drosophila. These cells are likely to be an integration center for the multiple sensory inputs that trigger male courtship.