Defining the catalytic activity of nanoceria in the P23H-1 rat, a photoreceptor degeneration model.
Defining the catalytic activity of nanoceria in the P23H-1 rat, a photoreceptor degeneration model.
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DOI：
10.1371/journal.pone.0121977
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
McGinnis JF
中科院分区：
文献类型：
--
作者：
Wong LL;Pye QN;Chen L;Seal S;McGinnis JF
Inorganic catalytic nanoceria or cerium oxide nanoparticles (CeNPs) are bona fide antioxidants that possess regenerative radical scavenging activities in vitro. Previously, we demonstrated that CeNPs had neuroprotective and anti-angiogenic properties in rodent retinal degeneration and neovascularization models. However, the cellular mechanisms and duration of the catalytic activity of CeNPs in preventing photoreceptor cell loss are still unknown. In this study, we sought to answer these questions using the P23H-1 rat, an autosomal dominant retinitis pigmentosa (adRP) model. A single dose of either saline or CeNPs was delivered intravitreally into the eyes of P23H-1 rats at 2–3 weeks of age. Retinal functions were examined at 3 to 7 weeks post injection. We quantified retinal proteins by Western blot analyses and counted the number of apoptotic (TUNEL+) profiles in the outer nuclear layer (ONL) of retinal sections. We measured free 8-isoprostanes to quantify lipid peroxidation in retinal tissues. We observed increased rod and cone cell functions up to three weeks post injection. Apoptotic cells were reduced by 46%, 56%, 21%, and 24% at 3, 7, 14, 21 days, respectively, after CeNPs injection compared to saline. Additionally, reduction of lipid peroxidation in the retinas of CeNPs-treated vs saline-treated animals was detected 14 days post injection. We validated that CeNPs were effective in delaying loss of photoreceptor cell function in an adRP rat model. This represents the fourth rodent retinal disease model that shows delay in disease progression after a single application of CeNPs. We further demonstrated that CeNPs slowed the rate of photoreceptor cell death. We deduced that the catalytic activity of CeNPs in vivo in this rat model to be undiminished for at least 7 days and then declined over the next 14 days after CeNPs administration.
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影响因子：
6.1
作者：
Kong, Li;Cai, Xue;Zhou, Xiaohong;Wong, Lily L.;Karakoti, Ajay S.;Seal, Sudipta;McGinnis, James F.
通讯作者：
McGinnis, James F.
DOI：
10.1083/jcb.119.3.493
发表时间：
1992-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gavrieli Y;Sherman Y;Ben-Sasson SA
通讯作者：
Ben-Sasson SA
影响因子：
14
作者：
Cai, Xue;Seal, Sudipta;McGinnis, James F.
通讯作者：
McGinnis, James F.
影响因子：
16.2
作者：
CHANG, GQ;HAO, Y;WONG, F
通讯作者：
WONG, F
影响因子：
82.9
作者：
Hollyfield, Joe G.;Bonilha, Vera L.;Perez, Victor L.
通讯作者：
Perez, Victor L.