Direct quantification of fatty acids in human milk by gas chromatography
Direct quantification of fatty acids in human milk by gas chromatography
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DOI：
10.1016/j.chroma.2013.01.094
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发表时间：
2013-04-05
影响因子：
4.1
通讯作者：
Destaillats, Frederic
中科院分区：
文献类型：
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作者：
Cruz-Hernandez, Cristina;Goeuriot, Sebastien;Destaillats, Frederic
母乳提供婴儿生长发育所必需的关键营养素。在过去的20年里，人们对母乳中的脂肪酸组成进行了广泛的研究，通过分析脂肪酸分布，然后进行脂质提取，并以每100克脂肪酸的克数表示数据，得出了结果。主要的缺点是，标准化的数据集不提供任何信息的脂肪酸母乳的量，因此婴儿的摄入量水平。本研究的目的是建立和验证一种直接分析人乳液体样品中脂肪酸含量的方法。以甲醇溶液中的盐酸为催化剂，十一酸甲酯（11:0）为内标，三癸醇（13:0 TAG）监测酯交换性能。脂肪酸甲酯（FAME）的分离使用100 m高极性毛细管柱和用于计算实验响应因子的认证校准混合物进行。该方法适用于250 μ L的人乳中脂肪酸的定量，可以用每分升人乳中脂肪酸的毫克数和脂肪酸的重量%表示。该方法经验证，重复性好[CV(r)]。
Human milk provides the key nutrients necessary for the infants' growth and development. The fatty acid composition of human milk has been extensively studied over the last 20 years and the results obtained by analyzing the fatty acid profile followed by lipid extraction and expressing data as g per 100 g of fatty acids. The main drawback is that normalizing data set does not give any information on the amount of fatty acid mother's milk and therefore the level of intake by the infant. The objective of the present study was to develop and validate a direct method to analyze the fatty acid content in liquid human milk samples. Hydrochloric acid in a solution of methanol was selected as the catalyst and methyl undecanoate (11:0) as the internal standard together with tritridecanoin (13:0 TAG) to monitor transesterification performance. The separation of fatty acid methyl esters (FAME) was performed using a 100 m highly polar capillary column and a certified calibration mixture used to calculate experimental response factors. The method is suitable to quantify fatty acids in human milk from a 250 mu L sample and allow expression of the data in mg of fatty acids per deciliter of human milk as well as weight % of fatty acids. The method has been validated and show a good repeatability [CV(r)