Micropore‐Forming Gelatin Methacryloyl (GelMA) Bioink Toolbox 2.0: Designable Tunability and Adaptability for 3D Bioprinting Applications
Micropore‐Forming Gelatin Methacryloyl (GelMA) Bioink Toolbox 2.0: Designable Tunability and Adaptability for 3D Bioprinting Applications
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Micropore™ 形成明胶甲基丙烯酰 (GelMA) Bioink Toolbox 2.0：针对 3D 生物打印应用的可设计可调性和适应性
DOI：
10.1002/smll.202106357
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发表时间：
2022
期刊：
影响因子：
13.3
通讯作者：
Hou, Linxi
中科院分区：
文献类型：
--
作者：
Yi, Sili;Liu, Qiong;Luo, Zeyu;He, Jacqueline Jialu;Ma, Hui‐Lin;Li, Wanlu;Wang, Di;Zhou, Cuiping;Garciamendez, Carlos Ezio;Hou, Linxi
众所周知，通常需要具有高度互连且尺寸可调的微孔的组织工程支架来促进细胞活力、运动性和功能。不幸的是，在 3D 生物打印应用中，通常缺乏以细胞相容的方式精确控制生物墨水内微孔结构的能力，直到最近报道了一种基于明胶甲基丙烯酰 (GelMA) 的微孔形成生物墨水方法。这种生物墨水利用了独特的水性两相乳液（ATPE）系统，其中聚环氧乙烷（PEO）液滴被用作致孔剂。考虑到与这一最初演示相关的局限性，本文通过对其他 GelMA 类型（猪和鱼，不同的甲基丙烯酰改性程度）和致孔剂类型（PEO、聚（乙烯醇）和葡聚糖）进行系统研究，以及致孔剂浓度和分子量对基于 GelMA 的 ATPE 生物墨水系统性能的影响，进一步加深了对微孔形成 GelMA 生物墨水的理解。本文不仅举例说明了可实现的显着更宽的微孔尺寸范围和更好的乳液稳定性，而且还证明了挤出和数字光处理生物打印的适用性得到了改善，并具有良好的细胞反应。
It is well‐known that tissue engineering scaffolds that feature highly interconnected and size‐adjustable micropores are oftentimes desired to promote cellular viability, motility, and functions. Unfortunately, the ability of precise control over the microporous structures within bioinks in a cytocompatible manner for applications in 3D bioprinting is generally lacking, until a method of micropore‐forming bioink based on gelatin methacryloyl (GelMA) was reported recently. This bioink took advantage of the unique aqueous two‐phase emulsion (ATPE) system, where poly(ethylene oxide) (PEO) droplets are utilized as the porogen. Considering the limitations associated with this very initial demonstration, this article has furthered the understanding of the micropore‐forming GelMA bioinks by conducting a systematic investigation into the additional GelMA types (porcine and fish, different methacryloyl‐modification degrees) and porogen types (PEO, poly(vinyl alcohol), and dextran), as well as the effects of the porogen concentrations and molecular weights on the properties of the GelMA‐based ATPE bioink system. This article exemplifies not only the significantly wider range of micropore sizes achievable and better emulsion stability, but also the improved suitability for both extrusion and digital light processing bioprinting with favorable cellular responses.