ADRENAL-STEROID RECEPTOR IMMUNOREACTIVITY IN CELLS BORN IN THE ADULT-RAT DENTATE GYRUS
ADRENAL-STEROID RECEPTOR IMMUNOREACTIVITY IN CELLS BORN IN THE ADULT-RAT DENTATE GYRUS
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DOI：
10.1016/0006-8993(93)90524-q
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发表时间：
1993-05-21
期刊：
影响因子：
2.9
通讯作者：
GOULD, E
中科院分区：
文献类型：
--
作者：
CAMERON, HA;WOOLLEY, CS;GOULD, E
多条证据表明，成年大鼠齿状回的细胞生成受肾上腺类固醇激素的调节。齿状回有丝分裂细胞肾上腺类固醇受体的表达支持这些激素直接作用于颗粒细胞前体的假设。我们进行了体内[H-3]胸腺嘧啶核苷放射自显影结合盐皮质激素受体(MR)和糖皮质激素受体(GR)免疫组织化学的存活时间进程，发现极少数[H-3]胸腺嘧啶核苷标记的有丝分裂细胞表达这些受体。注射[H-3]胸腺嘧啶核苷4周后，绝大多数[H-3]胸腺嘧啶核苷标记细胞呈MR和GR免疫反应阳性。这些结果表明，肾上腺类固醇不直接作用于成年大鼠齿状回中的颗粒细胞前体细胞。
Several lines of evidence indicate that cell birth in the adult rat dentate gyrus is regulated by adrenal steroids. The expression of adrenal steroid receptors by mitotic cells in the dentate gyrus would support the hypothesis that these hormones act directly on granule cell progenitors. We performed a survival time course of in vivo [H-3]thymidine autoradiography combined with immunohistochemistry for mineralocorticoid receptors (MR) and glucocorticoid receptors (GR) and found that very few [H-3]thymidine labeled mitotic cells express these receptors. By 4 weeks following [H-3]thymidine administration, the vast majority of [H-3]thymidine labeled cells were immunoreactive for MR and GR. These results suggest that adrenal steroids do not act directly on granule cell progenitors in the adult rat dentate gyrus.