Bioglass-Incorporated Methacrylated Gelatin Cryogel for Regeneration of Bone Defects
Bioglass-Incorporated Methacrylated Gelatin Cryogel for Regeneration of Bone Defects
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DOI：
10.3390/polym10080914
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发表时间：
2018-08-01
期刊：
影响因子：
5
通讯作者：
Hwang, Nathaniel S.
中科院分区：
文献类型：
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作者：
Kwon, Song;Lee, Seunghun S.;Hwang, Nathaniel S.
低温冷冻材料由于其固有的大孔结构而引起了组织工程领域的广泛关注。甲基丙烯酸明胶（GelMA）被认为适用于支架低温凝胶制造，是一种改性形式的明胶，因其保留细胞粘附部位的能力而受到重视。生物玻璃纳米颗粒也因其骨诱导和骨导电性而受到关注。在这里，我们制备了甲基丙烯酸明胶低温凝胶与不同浓度的生物玻璃纳米颗粒，研究其骨再生的潜力。在体外测试中，我们证明了低温凝胶中生物玻璃浓度的增加导致间充质细胞力学性能的改善和成骨分化的增强。此外，通过显微ct和组织学研究，小鼠颅骨缺损模型的体内实验表明，与其他测试组相比，加入最高浓度的生物玻璃纳米颗粒（2.5 w/w %）的GelMA低温凝胶诱导的骨形成最多。体外和体内实验结果突出了加入GelMA冷冻凝胶的生物玻璃纳米颗粒用于骨再生的潜力。
Cryogels have recently gained interest in the field of tissue engineering as they inherently possess an interconnected macroporous structure. Considered to be suitable for scaffold cryogel fabrication, methacrylated gelatin (GelMA) is a modified form of gelatin valued for its ability to retain cell adhesion site. Bioglass nanoparticles have also attracted attention in the field due to their osteoinductive and osteoconductive behavior. Here, we prepare methacrylated gelatin cryogel with varying concentration of bioglass nanoparticles to study its potential for bone regeneration. We demonstrate that an increase in bioglass concentration in cryogel leads to improved mechanical property and augmented osteogenic differentiation of mesenchymal cells during in vitro testing. Furthermore, in vivo testing in mice cranial defect model shows that highest concentration of bioglass nanoparticles (2.5 w/w %) incorporated in GelMA cryogel induces the most bone formation compared to the other tested groups, as studied by micro-CT and histology. The in vitro and in vivo results highlight the potential of bioglass nanoparticles incorporated in GelMA cryogel for bone regeneration.