Dorsalization of the neural tube by the non-neural ectoderm.
Dorsalization of the neural tube by the non-neural ectoderm.
复制标题
DOI：
--
复制
发表时间：
1995-07
期刊：
影响因子：
4.6
通讯作者：
Mary E. Dickinson;M. Selleck;Andrew P. McMahon;M. Bronner‐Fraser
中科院分区：
文献类型：
--
作者：
Mary E. Dickinson;M. Selleck;Andrew P. McMahon;M. Bronner‐Fraser
沿着脊髓背腹轴的细胞类型的图案需要一组复杂的诱导信号。虽然脊索中胚层是一个众所周知的腹侧信号源，但对诱导背侧细胞类型（包括神经嵴）的线索知之甚少。在这里，我们证明，并列的非神经和神经外胚层是足以诱导背标记物，Wnt-1，Wnt-3a和鼻涕虫的表达，以及神经嵴细胞的形成。此外，神经板表达Wnt-1和Wnt-3a的能力似乎是阶段依赖性的，仅当神经组织取自8-10期胚胎而不是取自4期胚胎时发生，与非神经外胚层的年龄无关。与Wnt基因表达的诱导相反，当将第4阶段或第8-10阶段神经板与非神经外胚层接触时，可以诱导神经嵴细胞形成和Slug表达。这些数据表明，非神经外胚层提供了一个信号（或多个信号），指定神经管内的背细胞类型，并且该反应取决于神经组织的能力。
The patterning of cell types along the dorsoventral axis of the spinal cord requires a complex set of inductive signals. While the chordamesoderm is a well-known source of ventralizing signals, relatively little is known about the cues that induce dorsal cell types, including neural crest. Here, we demonstrate that juxtaposition of the non-neural and neural ectoderm is sufficient to induce the expression of dorsal markers, Wnt-1, Wnt-3a and Slug, as well as the formation of neural crest cells. In addition, the competence of neural plate to express Wnt-1 and Wnt-3a appears to be stage dependent, occurring only when neural tissue is taken from stage 8-10 embryos but not from stage 4 embryos, regardless of the age of the non-neural ectoderm. In contrast to the induction of Wnt gene expression, neural crest cell formation and Slug expression can be induced when either stage 4 or stage 8-10 neural plates are placed in contact with the non-neural ectoderm. These data suggest that the non-neural ectoderm provides a signal (or signals) that specifies dorsal cell types within the neural tube, and that the response is dependent on the competence of the neural tissue.