Effect of confinement on DNA dynamics in microfluidic devices
Effect of confinement on DNA dynamics in microfluidic devices
复制标题
DOI：
10.1063/1.1575200
复制
发表时间：
2003-07-08
影响因子：
4.4
通讯作者：
de Pablo, JJ
中科院分区：
文献类型：
--
作者：
Jendrejack, RM;Schwartz, DC;de Pablo, JJ
溶解的长链大分子在高受限环境中的动力学与在散装溶液中的动力学不同。本文提出了一种计算方法来详细预测这些动力学，并将其应用于类似于1-100 mum DNA在微米尺度通道中的行为。该方法由聚合物动力学的自一致粗粒度朗之万描述和聚合物段运动产生的流动的数值解组成。扩散系数和最长弛豫时间表现出从自由解到约束行为的广泛交叉，其中心为点Happroximate to 10s (b)，其中H为通道宽度，S-b为旋转的自由解链半径。在大通道中，扩散率类似于沿孔中心线扩散的球体。对于高约束链(H/S-b
The dynamics of dissolved long-chain macromolecules are different in highly confined environments than in bulk solution. A computational method is presented here for detailed prediction of these dynamics, and applied to the behavior of similar to1-100 mum DNA in micron-scale channels. The method is comprised of a self-consistent coarse-grained Langevin description of the polymer dynamics and a numerical solution of the flow generated by the motion of polymer segments. Diffusivity and longest relaxation time show a broad crossover from free-solution to confined behavior centered about the point Happroximate to10S(b), where H is the channel width and S-b is the free-solution chain radius of gyration. In large channels, the diffusivity is similar to that of a sphere diffusing along the centerline of a pore. For highly confined chains (H/S-b