Forkhead box C1 promoter upstream transcript, a novel long non-coding RNA, regulates proliferation and migration in basal-like breast cancer
Forkhead box C1 promoter upstream transcript, a novel long non-coding RNA, regulates proliferation and migration in basal-like breast cancer
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DOI：
10.3892/mmr.2014.3089
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发表时间：
2015-04-01
影响因子：
3.4
通讯作者：
Geng, Peiliang
中科院分区：
文献类型：
--
作者：
Liu, Juntao;Shen, Lei;Geng, Peiliang
最近的研究表明，长链非编码RNA（lncRNA）在肿瘤发生中具有重要的调控作用。叉头盒C1（FOXC 1）是基底细胞样乳腺癌（BLBC）的重要癌相关基因。在本研究中，一种新的lncRNA，FOXC 1启动子上游转录（FOXCUT）在BLBC患者使用聚合酶链反应分析进行了研究。结果显示FOXCUT和FOXC 1的表达呈正相关。当FOXCUT的表达被小干扰RNA下调时，FOXC 1的表达也同样降低。此外，在MDA-MB-231和MDA-MB-468乳腺癌细胞中，FOXCUT的敲低在体外显著抑制细胞增殖和迁移。总之，FOXCUT lncRNA可能通过调节其配对mRNA FOXC 1在BLBC的肿瘤进展中起作用，表明FOXCUT可能作为BLBC中的新生物标志物和治疗靶点。
Recent studies have shown that long non-coding RNAs (lncRNAs) have crucial regulating roles in carcinogenesis. Forkhead box C1 (FOXC1) is an important cancer-associated gene in basal-like breast cancer (BLBC). In the present study, a novel lncRNA, FOXC1 promoter upstream transcript (FOXCUT) was investigated in BLBC patients using polymerase chain reaction analysis. The results showed that the expression of FOXCUT and FOXC1 were positively correlated. When the expression of FOXCUT was downregulated by small interfering RNA, the expression of FOXC1 was similarly reduced. Furthermore, in MDA-MB-231 and MDA-MB-468 breast cancer cells, knockdown of FOXCUT markedly inhibited cell proliferation and migration in vitro. In conclusion, FOXCUT lncRNA may be functionally involved in the tumor progression of BLBCs through the regulation of its paired mRNA, FOXC1, demonstrating that FOXCUT may serve as a novel biomarker and therapeutic target in BLBCs.