An optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton
An optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton
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DOI：
10.1007/bf02702563
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发表时间：
2004-03-01
影响因子：
2.9
通讯作者：
Chen, F
中科院分区：
文献类型：
--
作者：
Cai, YF;Zhang, H;Chen, F
采用优化的高效液相色谱法，在C-18柱（4（.）6 mm x 250 mm，5 μ m颗粒）与甲醇-0（.）5%乙酸水溶液，90：10（v/v），作为移动的流动相，流速为0（.）8 ml/min，254 nm紫外检测。该方法具有高度重现性，精密度[相对标准偏差（RSD）]和准确度[相对平均误差（RME）]均为94%。结果表明，不同腺体类型的棉花品种（种）间差异较大，包括特殊无腺体和普通有腺体的陆地棉、陆地棉、棉叶、叶、棉叶、棉叶、叶、叶并展示了珍贵的非凡棉花的不同腺体资源。
A comparative study on gossypol content of various genetic types of pigment glands of cotton varieties was conducted through an optimized high-performance liquid chromatography (HPLC) on a C-18 column (4(.)6 mm x 250 mm, 5 mum particle) with methanol-0(.)5% acetic acid aqueous solution, 90 : 10 (v/v), as mobile phase, at a flow rate of 0(.)8 ml/min and UV detection at 254 nm. The method was shown to be highly reproducible, with precision [as relative standard deviation (RSD)] and accuracy [as relative mean error (RME)] 94%. The results revealed major differences among the different gland varieties or species of cotton, including the special and ordinary glandless and glanded Gossypium hirsutum, G. barbadense, and displayed the precious resources of different glands of extraordinary cotton.