COLLAGENOLYTIC ACTIVITY IN AMPHIBIAN TISSUES - A TISSUE CULTURE ASSAY
COLLAGENOLYTIC ACTIVITY IN AMPHIBIAN TISSUES - A TISSUE CULTURE ASSAY
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DOI：
10.1073/pnas.48.6.1014
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发表时间：
1962-01-01
影响因子：
11.1
通讯作者：
LAPIERE, CM
中科院分区：
文献类型：
--
作者：
GROSS, J;LAPIERE, CM
在培养的活动物组织中，证明了在中性pH和生理温度下作用于未变性胶原的可扩散的胶原溶解因子的产生。牛蛙蝌蚪组织（皮肤，肠道和鳃）的热重建的中性小牛和豚鼠皮肤胶原蛋白凝胶的培养降解的底物可透析的胶原蛋白肽。该底物不受常见的蛋白水解酶、组织蛋白酶C、蝌蚪组织提取物或杀死的蝌蚪组织的攻击。所用的实验系统可以发展成小组织碎片中胶原溶解活性的定量测定。
Produc-tion of a diffusible collagenolytic factor acting at neutral pH and physiologic temperature on undenatured collagen was demonstrated in living animal tissues in culture. Cultivation of bullfrog tadpole tissues (skin, gut, and gills) on thermally reconstituted neutral calf and guinea pig skin collagen gels degraded the substrate to dialyzable collagen peptides. This substrate was not attacked by the common proteolytic enzymes, by Cathepsin C, by tadpole tissue extracts, or by killed tadpole tissue. The experimental system used may be developed into a quantitative assay for collagenolytic activity in small tissue fragments.