Influence of norepinephrine and catecholamine-depleting agents on the synthesis and release of prolactin and growth hormone.
Influence of norepinephrine and catecholamine-depleting agents on the synthesis and release of prolactin and growth hormone.
复制标题
去甲肾上腺素和儿茶酚胺消耗剂对催乳素和生长激素合成和释放的影响。
作者：
R. Macleod
研究了亮氨酸-4,5-3H 体外掺入大鼠垂体及其随后释放到培养介质中的情况。催乳素和生长激素的定量是通过测量聚丙烯酰胺凝胶技术获得的条带的强度而无需生物测定。大部分新合成的催乳素被释放到培养基中，而不是保留在腺体内。添加去甲肾上腺素 (10-6M) 会导致腺体在 7 小时孵育期间释放的放射性催乳素量减少 70-85%。标记的催乳素在处理的腺体中积累，表明去甲肾上腺素的主要作用是减少催乳素的释放。然而，积累量与释放抑制程度不成正比，表明催乳素合成受到二次抑制。添加去甲肾上腺素30分钟后可检测到腺体中放射性催乳素的增加，并且30分钟后具有统计学意义。相似的...
The in vitro incorporation of leucine- 4,5-3H into rat pituitary glands and its subsequent release into the incubation medium were studied. Quantitation of prolactin and growth hormone was by measurement of intensity of the bands obtained with the polyacrylamide gel technique without bioassay. Most of this newly synthesized prolactin is released into the medium and not retained within the gland. The addition of norepinephrine (10-6M) caused a 70–85% decrease in the amount of radioactive prolactin released by the gland during a 7-hr incubation. An accumulation of labeled prolactin occurred in the treated glands, indicating the primary action of norepinephrine was to decrease prolactin release. The accumulation, however, was not proportional to the degree of inhibition of release, indicating that prolactin synthesis was secondarily inhibited. An increase in the radioactive prolactin in the gland was detectable 30 min afterthe addition of norepinephrine and was statistically significant 30 min later. Similar...