Determination of aromatic amines in food products and composite food packaging bags by capillary electrophoresis coupled with transient isotachophoretic stacking
Determination of aromatic amines in food products and composite food packaging bags by capillary electrophoresis coupled with transient isotachophoretic stacking
复制标题
毛细管电泳结合瞬态等速电泳堆积法测定食品和复合食品包装袋中的芳香胺
DOI：
10.1016/j.chroma.2009.05.089
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发表时间：
2009-10-23
影响因子：
4.1
通讯作者：
Chen, Yi
中科院分区：
文献类型：
--
作者：
Wang, Xiaoyu;Chen, Yi
建立了毛细管电泳法测定食品中芳香胺的方法。与一个在线耦合的瞬态等速电泳堆积的方法，该方法已经产生了约200倍的灵敏度提高，在紫外检测的三个伯芳香胺和三聚氰胺。以K+为先导离子，Tris（+）为终止离子，将10 cm（相当于0.44 μ L）的样品溶液注入60 cm（有效长度50 cm）的毛细管中进行分离，检测限低至2.0 × 10（-8）M。在10 min内实现基线分离，迁移时间的相对标准偏差为0.41-0.75%（日内）或1.2-1.5%（日间），峰面积的相对标准偏差为3.8-4.3%（日内）或5.2-6.7%（日间）。方法可直接用于奶粉中三聚氰胺的测定，回收率在92.0%~ 107.1%之间。经10倍离线富集后，该方法也可用于复合食品包装袋中痕量伯芳胺的分析，检出限小于1 μ g/L。(C)2009爱思唯尔有限公司版权所有。
A capillary electrophoretic method was explored to assay aromatic amines in food samples. With an inline-coupled transient isotachophoretic stacking approach, the method has yielded about 200-fold improvement of sensitivity in UV detection of three primary aromatic amines and melamine. By using K+ as a leading ion and Tris(+) as a terminating ion, a plug of 10 cm (equivalent to 0.44 mu L) sample solution was allowed to introduce into a 60 cm (50 cm effective) capillary for separation, giving limits of detection down to 2.0 x 10(-8) M. Baseline separation was achieved within 10 min, with relative standard deviation of 0.41-0.75% (intra-day) or 1.2-1.5% (inter-day) for migration time and 3.8-4.3% (intra-day) or 5.2-6.7% (inter-day) for peak area. The method was directly applicable to assaying the melamine in powder milk samples, with recovery in between 92.0% and 107.1%. The method could also be applied to the analysis of trace primary aromatic amines migrating from composite food packaging bags after combination of a 10-fold off-line concentration step, with limit of detection down to less than 1 mu g/L By this method, 4,4'-diaminophenylmethane and 2,4-diaminotoluene were thus found in three types of composite food packaging bags. (C) 2009 Elsevier B.V. All rights reserved.