Investigation of mechanisms of bendiocarb resistance in Anopheles gambiae populations from the city of Yaoundé, Cameroon.
Investigation of mechanisms of bendiocarb resistance in Anopheles gambiae populations from the city of Yaoundé, Cameroon.
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DOI：
10.1186/s12936-016-1483-3
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发表时间：
2016-08-22
期刊：
影响因子：
3
通讯作者：
Wondji CS
中科院分区：
文献类型：
--
作者：
Antonio-Nkondjio C;Poupardin R;Tene BF;Kopya E;Costantini C;Awono-Ambene P;Wondji CS
喀麦隆雅温得市的冈比亚按蚊种群中出现了对氨基甲酸酯杀虫剂恶虫威的抗药性。然而，这种耐药性的分子基础仍未得到表征。本研究的目的是探讨抗虫威的机制。gambia冈比亚populations人口from Yaoundé雅温得.安氏杆菌的易感性水平。冈比亚公司2010年至2013年，使用生物测定法评估了0.1%的恶虫威。使用TaqMan分析筛选对苯虫威具有抗性的蚊子、未暴露的蚊子和易感蚊子中Ace-1 R突变的存在。进行微阵列分析以评估耐药、未暴露和敏感之间差异表达的基因的模式。与来自污染和未污染地点的蚊子相比，来自种植地点的蚊子对苯虫威的抗性更为普遍。两个都是。冈比亚按蚊和科氏按蚊对恶虫威表现出抗药性。未检测到G119 S突变，表明抗性主要是代谢性的。基因芯片分析显示，CYP 6 z3、CYP 6 z1、CYP 12 f2、CYP 6 m3和CYP 6p 4等几种细胞色素P450 s基因的过表达。基因本体论（GO）富集分析支持细胞色素P450的解毒作用，与P450活性相关的几个GO术语在抗性样品中显著富集。其他解毒基因包括UDP-葡萄糖基转移酶、谷胱甘肽-S转移酶和ABC转运蛋白。这项研究强调了代谢机制在安虫威抗性中的可能意义。该研究对雅温得的冈比亚种群进行了研究，并强调需要进一步研究，以验证参与这种抗性的解毒基因的功能。
Resistance to the carbamate insecticide bendiocarb is emerging in Anopheles gambiae populations from the city of Yaoundé in Cameroon. However, the molecular basis of this resistance remains uncharacterized. The present study objective is to investigate mechanisms promoting resistance to bendiocarb in An. gambiae populations from Yaoundé. The level of susceptibility of An. gambiae s.l. to bendiocarb 0.1 % was assessed from 2010 to 2013 using bioassays. Mosquitoes resistant to bendiocarb, unexposed and susceptible mosquitoes were screened for the presence of the Ace-1R mutation using TaqMan assays. Microarray analyses were performed to assess the pattern of genes differentially expressed between resistant, unexposed and susceptible. Bendiocarb resistance was more prevalent in mosquitoes originating from cultivated sites compared to those from polluted and unpolluted sites. Both An. gambiae and Anopheles coluzzii were found to display resistance to bendiocarb. No G119S mutation was detected suggesting that resistance was mainly metabolic. Microarray analysis revealed the over-expression of several cytochrome P450 s genes including cyp6z3, cyp6z1, cyp12f2, cyp6m3 and cyp6p4. Gene ontology (GO) enrichment analysis supported the detoxification role of cytochrome P450 s with several GO terms associated with P450 activity significantly enriched in resistant samples. Other detoxification genes included UDP-glucosyl transferases, glutathione-S transferases and ABC transporters. The study highlights the probable implication of metabolic mechanisms in bendiocarb resistance in An. gambiae populations from Yaoundé and stresses the need for further studies leading to functional validation of detoxification genes involved in this resistance.