Bioluminescence-Optogenetics: A Practical Guide.
Bioluminescence-Optogenetics: A Practical Guide.
复制标题
生物发光-光遗传学：实用指南。
DOI：
10.1007/978-1-0716-2473-9_26
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Gross,RobertE
中科院分区：
文献类型：
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作者：
Stern,MatthewA;Skelton,Henry;Fernandez,AlejandraM;Gutekunst,Claire-AnneN;Berglund,Ken;Gross,RobertE
通过遗传技术控制神经活动是神经科学领域的重要工具，也是治疗大量神经和精神疾病的潜在途径。在这里，我们描述了一种新兴的分子神经调节方法，称为生物发光光遗传学（BL- og），其中BL被利用来激活细菌光驱动通道和神经元中表达的泵来控制它们的活动。BL-OG是通过称为发光蛋白（LMOs）的视蛋白-荧光素酶融合蛋白实现的。在本章中，我们将使用细胞系和原代培养细胞在体外应用BL-OG和LMOs提供实用指南。在下一章中，我们将把重点转向BL-OG在离体和体内啮齿动物神经系统模型中的应用。
Manipulation of neural activity in genetically predefined populations of neurons through genetic techniques is an essential tool in the field of neuroscience as well as a potential avenue in treating a vast assortment of neurological and psychiatric diseases. Here, we describe an emerging methodology of molecular neuromodulation termed bioluminescence-optogenetics (BL-OG) where BL is harnessed to activate bacterial light-driven channels and pumps expressed in neurons to control their activity. BL-OG is realized through opsin-luciferase fusion proteins called luminopsins (LMOs). In this chapter, we will provide a practical guide for applying BL-OG and LMOs in vitro using a cell line and primary cells in culture. In the following chapter, we will turn our focus towards BL-OG applications in ex vivo and in vivo rodent models of the nervous system.