Linking alkaline phosphatase activity with bacterial phoD gene abundance in soil from a long-term management trial
Linking alkaline phosphatase activity with bacterial phoD gene abundance in soil from a long-term management trial
复制标题
DOI：
10.1016/j.geoderma.2014.10.016
复制
发表时间：
2015-11-01
期刊：
影响因子：
6.1
通讯作者：
Dunfield, Kari E.
中科院分区：
文献类型：
--
作者：
Fraser, Tandra;Lynch, Derek H.;Dunfield, Kari E.
土地管理实践的变化可能对有机P离职重要的土壤微生物群落具有重大影响。土壤细菌可以通过排泄磷酸酶催化酯 - 磷酸键水解的磷酸酶来增加植物P的可利用率。检查植入细菌的碱性磷酸酶基因的多样性和丰度可以为土壤中碱性磷酸酶产生的宝贵见解。这项研究检查了20年没有输入有机（ORG），有机肥料（ORG + M），常规（Cons）和恢复的草原（PRA）管理对土壤P生物利用度，碱性磷酸酶活性（ALP）和ALP基因（PHOD）的丰度和多样性在加拿大北部大平原的土壤中产生细菌。管理系统影响了生物利用的P（p <0.001），但不影响总P，在ORG系统中浓度最低，而PRA的浓度最低。在ORG和ORG + M处理中观察到较高的ALP发生率，2011年生物利用P和ALP之间存在显着的负相关性（R（2）= 0.71; P = 0.03）和2012年（R（2）= 0.51; P = = 0.51; P = 0.02），表明在限制条件下ALP活性增加。 PHOD基因丰度在组织和组织 + M中也最高，导致细菌pHOD丰度与ALP活性之间存在显着的正相关关系（R（2）= 0.71； P = 0.009）。对PHOD细菌群落指纹的分析表明，与ORG和ORG + M相比，CORV中的物种数量更高，这与在组织管理下考虑更大的ALP活性相反。在2012年，ORG + M的带状谱显示第二种粪便后显示出较少的PHOD细菌物种，尽管ALP活性高于2011年。这表明一些物种可能会产生更多的ALP，而定量基因分析是更好的指标。活动比存在的物种数量。 （c）2014 Elsevier B.V.保留所有权利。
Changes in land management practices may have significant implications for soil microbial communities important in organic P turnover. Soil bacteria can increase plant P availability by excreting phosphatase enzymes which catalyze the hydrolysis of ester-phosphate bonds. Examining the diversity and abundance of alkaline phosphatase gene harboring bacteria may provide valuable insight into alkaline phosphatase production in soils. This study examined the effect of 20 years of no input organic (ORG), organic with composted manure (ORG + M), conventional (CONV) and restored prairie (PRA) management on soil P bioavailability, alkaline phosphatase activity (ALP), and abundance and diversity of ALP gene (phoD) harboring bacteria in soils from the northern Great Plains of Canada. Management system influenced bioavailable P (P < 0.001), but not total P, with the lowest concentrations in the ORG systems and the highest in PRA. Higher rates of ALP were observed in the ORG and ORG + M treatments with a significant negative correlation between bioavailable P and ALP in 2011 (r(2) = 0.71; P = 0.03) and 2012 (r(2) = 0.51; P = 0.02), suggesting that ALP activity increased under P limiting conditions. The phoD gene abundance was also highest in ORG and ORG + M resulting in a significant positive relationship between bacterial phoD abundance and ALP activity (r(2) = 0.71; P = 0.009). Analysis of phoD bacterial community fingerprints showed a higher number of species in CONV compared to ORG and ORG + M, contrary to what was expected considering greater ALP activity under ORG management. In 2012, banding profiles of ORG + M showed fewer phoD bacterial species following the second manure application, although ALP activity is higher than in 2011. This indicates that a few species may be producing more ALP and that quantitative gene analysis was a better indicator of activity than the number of species present. (C) 2014 Elsevier B.V. All rights reserved.