Addition of ascorbic acid solution to stored murine red blood cells increases posttransfusion recovery and decreases microparticles and alloimmunization
Addition of ascorbic acid solution to stored murine red blood cells increases posttransfusion recovery and decreases microparticles and alloimmunization
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DOI：
10.1111/trf.12106
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发表时间：
2013-10-01
期刊：
影响因子：
2.9
通讯作者：
Yazer, Mark H.
中科院分区：
文献类型：
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作者：
Stowell, Sean R.;Smith, Nicole H.;Yazer, Mark H.
背景红细胞 (RBC) 的储存会导致许多变化，随着时间的推移，这些变化会导致输注红细胞的恢复率下降。此外（至少在动物模型中），储存的红细胞具有更高的免疫原性，并且还能刺激输血受者体内炎症细胞因子的全身释放。红细胞储存损伤的组成部分之一是氧化损伤的积累。我们测试了这样的假设：在储存的红细胞中添加化学抗氧化剂（抗坏血酸）会提高储存的红细胞的质量。研究设计和方法从表达红细胞特异性模型转基因 (HOD) 的 FVB.HOD 小鼠中收获红细胞，并用盐水中的抗坏血酸或单独的盐水储存 14 天。通过流式细胞术追踪输血后二十四小时红细胞的恢复情况。通过流式细胞仪交叉配血监测同种免疫。通过多重珠阵列监测细胞因子。结果在标准条件下储存的红细胞在输血后24小时恢复有所下降，并增加了小鼠受体中同种抗体和白细胞介素（IL）-6和单核细胞趋化蛋白（MCP）-1分泌的诱导。添加抗坏血酸从 3.6 至 10.8 mmol/L 导致微粒形成显着减少，输血后 24 小时红细胞恢复得到改善 (p
BackgroundThe storage of red blood cells (RBCs) results in numerous changes, which over time result in decreased recovery of transfused RBCs. In addition (at least in animal models), stored RBCs can be more immunogenic and also stimulate the systemic release of inflammatory cytokines in transfusion recipients. One component of the RBC storage lesion is the accumulation of oxidative damage. We tested the hypothesis that adding a chemical antioxidant (ascorbic acid) to stored RBCs would improve the quality of the stored RBCs.Study Design and MethodsRBCs were harvested from FVB.HOD mice that express an RBC-specific model transgene (HOD) and stored for 14 days with either ascorbic acid in saline or saline alone. Twenty-four-hour posttransfusion recovery of RBCs was tracked by flow cytometry. Alloimmunization was monitored by flow cytometry crossmatch. Cytokines were monitored by multiplex bead arrays.ResultsRBCs stored under standard conditions had decreased 24-hour posttransfusion recovery and increased induction of both alloantibodies and interleukin (IL)-6 and monocyte chemoattractant protein (MCP)-1 secretion in the mouse recipients. Addition of ascorbic acid from 3.6 to 10.8mmol/L resulted in a significant decrease in microparticle formation, an improved RBC 24-hour posttransfusion recovery (p