Massive expansion of antigen-specific CD8+ T cells during an acute virus infection
Massive expansion of antigen-specific CD8+ T cells during an acute virus infection
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DOI：
10.1016/s1074-7613(00)80469-0
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发表时间：
1998-02-01
期刊：
影响因子：
32.4
通讯作者：
Bevan, MJ
中科院分区：
文献类型：
--
作者：
Butz, EA;Bevan, MJ
在LCMV感染过程中，CD8（+）T细胞大量扩增。旁观者激活被认为起作用，因为在有限稀释分析中很少有细胞评分为LCMV特异性。相反，我们发现至少四分之一的CD8（+）细胞分泌IFN γ特异性地响应于LCMV肽在响应的峰值。此外，通过分析过继转移的LCMV特异性TCR转基因CD8（+）T细胞在同源宿主中的扩增，我们已经确定大多数CD8（+）细胞扩增是病毒特异性的。单特异性TCR转基因T细胞对宿主对三个LCMV表位的反应的影响的分析表明，CTL前体以表位特异性的方式竞争APC上的位点，并且这种竞争决定了反应的特异性。
During LCMV infection, CD8(+) T cells expand greatly. Bystander activation has been thought to play a role because few cells score as LCMV specific in limiting dilution analysis. In contrast, we find that at least a quarter of the CD8(+) cells secrete IFN gamma specifically in response to LCMV peptides at the peak of the response. Moreover, by analyzing the expansion of adoptively transferred LCMV-specific, TCR-transgenic CD8(+) T cells in congenic hosts, we have determined that most of the CD8(+) cell expansion is virus specific. Analysis of the effect of the monospecific TCR-transgenic T cells on the host response to three LCMV epitopes suggests that CTL precursors compete for sites on the APC in an epitope-specific fashion and that this competition determines the specificity of the response.