Phosphate and vanadate reduce the efficiency of the chemo-mechanical energy transformation in cardiac muscle.
Phosphate and vanadate reduce the efficiency of the chemo-mechanical energy transformation in cardiac muscle.
复制标题
磷酸盐和钒酸盐降低心肌中化学机械能转化的效率。
DOI：
10.1007/978-1-4684-4259-5_33
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发表时间：
1982
影响因子：
--
通讯作者：
Rüegg,JC
中科院分区：
文献类型：
--
作者：
Herzig,JW;Peterson,JW;Solaro,RJ;Rüegg,JC
通过用Lubrol WX和甘油处理，将猪心右心室的小梁制备物“剥皮”。钙++激活的等长收缩逐渐放松的无机磷酸盐（Pi）在毫摩尔范围内或钒酸盐（Vi）在微摩尔范围内，而张力成本（ATP分裂/产生的力）增加了1.75倍。从测量力，ATP酶活性，即时刚度和拉伸激活，提供的证据表明，机械失活和张力成本的增加可能会导致从肌球蛋白跨桥周期的加速，由于直接干扰的Pi和Vi与化学机械能量转换在收缩蛋白。这种机制在心力衰竭或肌肉疲劳的可能意义进行了讨论。
Trabecular preparations from the hog heart right ventricle were “skinned” by treatment with Lubrol WX and glycerol. Ca++activated isometric contractions were gradedly relaxed by inorganic phosphate (Pi) in the millimolar range or vanadate (Vi) in the micromolar range while tension cost (ATP split/force generated) was increased by a factor of 1.75. From measurements of force, ATPase activity, immediate stiffness and stretch activation, evidence is provided that the mechanical deactivation and the increase in tension cost may result from an acceleration of the myosin cross-bridge cycle, due to a direct interference of Piand Viwith the chemomechanical energy transformation at the contractile proteins. The possible significance of such a mechanism in cardiac failure or muscle fatigue is discussed.