Effect of inulin supplementation on selected gastric, duodenal, and caecal microbiota and short chain fatty acid pattern in growing piglets
Effect of inulin supplementation on selected gastric, duodenal, and caecal microbiota and short chain fatty acid pattern in growing piglets
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DOI：
10.1080/17450390701431631
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发表时间：
2007-01-01
影响因子：
2
通讯作者：
Metges, Cornelia C.
中科院分区：
文献类型：
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作者：
Eberhard, Markus;Hennig, Ulf;Metges, Cornelia C.
本研究探讨了生长猪日粮和菊粉摄入量对生长猪上肠道和盲肠中双歧杆菌和乳酸菌数量及其他精选细菌的影响。从断奶后2周(28d)开始，72头猪分别饲喂两种类型的日粮(小麦/大麦(WB)或玉米/面筋(MG))，不添加或添加3%菊粉(WB+I，MG+I)，分别饲喂3周和6周。用荧光原位杂交法对肠道细菌进行定量(n=8/组)。喂养时间对所测试的变量没有影响，所以两个时期的数据都被合并了。胃细菌总数为log(10)7.4个/g消化液。断奶后两周，胃和十二指肠中检测到双歧杆菌，此后双歧杆菌消失。在空肠和盲肠中，双歧杆菌的含量为log(10)7.0个/g消化液。菊粉对乳杆菌、双歧杆菌、肠球菌、肠杆菌和球状芽孢杆菌/真细菌组的细菌数量没有影响。MG日粮的胃肠菊糖消失率(73.7vs.60.7%，P=0.013)高于对照组(P<0.05)。添加菊粉的日粮盲肠乙酸酯含量较低(p<；0.05)，而饲喂WB日粮的猪的丙酸和丁酸含量较高(p<；0.05)。WB组盲肠总短链脂肪酸浓度较高，pH值较低(p<；0.05)。
We explored whether bifidobacteria and lactobacilli numbers and other selected bacteria in the upper intestine and the caecum of growing pigs were affected by diet and intake of inulin. Starting at two weeks after weaning ( 28 d) 72 pigs were fed two types of diets ( wheat/ barley ( WB) or maize/ gluten ( MG)), without or with 3% inulin ( WB + I, MG + I)) for three and six weeks. Intestinal bacteria were quantified by fluorescence- in- situ- hybridization ( n = 8/ group). Duration of feeding had no effect on the variables tested, so data for both periods were pooled. Gastric total bacteria amounted to log(10) 7.4/ g digesta. Bifidobacteria were detected in stomach and duodenum two weeks after weaning and disappeared thereafter. In jejunum and caecum bifidobacteria were present at a level of log(10) 7.0/ g digesta. Inulin did not alter numbers of lactobacilli, bifidobacteria, enterococci, enterobacteria and bacteria of the Clostridium coccoides/ Eubacterium rectale- group. Inulin disappearance in stomach plus jejunum was higher with the MG diet ( 73.7 vs. 60.7%, p = 0.013). Caecal acetate was lower in inulin- supplemented diets ( p < 0.05) whereas propionate and butyrate were higher in pigs fed the WB diets ( p < 0.05). With the WB diet total caecal short chain fatty acids concentration was higher which resulted in a lower pH value ( p < 0.05).