Serum interleukin-12 levels in patients with gastric cancer
Serum interleukin-12 levels in patients with gastric cancer
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DOI：
10.1007/s00595-004-2860-z
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发表时间：
2004-12-01
期刊：
影响因子：
2.5
通讯作者：
Hirayama, R
中科院分区：
文献类型：
--
作者：
Murakami, S;Okubo, K;Hirayama, R
目的。为了解胃癌患者手术前的免疫状态，我们探讨了血清白介素12(IL-12)水平与临床病理因素的关系。采用人IL-12+p40免疫试剂盒双抗体夹心酶联免疫吸附试验检测127例胃癌患者和35例健康人血清IL-12水平。胃癌患者血清IL-12水平显著高于健康对照组(P<；0.05)。T4组患者血清IL-12水平显著低于T1、T2、T3型患者(P<0.01)。血清IL-12水平在有无淋巴结、肝脏或腹膜转移者与无转移者之间无显著差异。有远处转移者血清IL-12水平显著低于无远处转移者(P<；0.02)。两组患者血清IL-12水平按病理组织学分级无显著差异。直线相关分析显示，血清IL-12与癌胚抗原、糖类抗原(CA)19-9、CA72-4、甲胎蛋白、免疫抑制酸性蛋白无显著相关性。而血清IL-12水平与可溶性IL-2受体水平呈显著正相关(r=0.53，P<；0.01)。进展期胃癌患者血清IL-12水平明显低于进展期胃癌患者。这是因为晚期癌症患者的巨噬细胞会非常活跃，无法产生足够的IL-12。
Purpose. To evaluate the immunological status of patients with gastric cancer before surgery, we investigated the relationship between serum interleukin-12 (IL-12) levels and clinicopathological factors.Methods. We measured serum IL-12 levels in 127 patients with gastric cancer and 35 healthy controls, by a sandwich enzyme-linked immunosorbent assay using the Human IL-12 +p40 Immunoassay kit.Results. The serum IL-12 levels in the patients with gastric cancer were significantly higher than those of the healthy controls (P < 0.05). There were no significant differences in disease stage or gross appearance among the cancer groups, but the serum IL-12 levels in patients with T4 disease were significantly lower than those in patients with T1, T2, or T3 (P < 0.01). There were no significant differences in serum IL-12 levels between patients with and those without lymph node, liver, or peritoneal metastasis. The serum IL-12 levels in patients with distant metastasis were significantly lower than those in patients without distant metastasis (P < 0.02). There were no significant differences in the serum IL-12 levels according to classification by histopathological findings. Analysis with the linear correlation coefficient showed no significant correlation between serum IL-12 and serum carcinoembryonic antigen, carbohydrate antigen (CA) 19-9, CA 72-4, alpha-fetoprotein, or immunosuppressive acidic protein. However, there was a significant relationship between serum IL-12 levels and soluble IL-2 receptor levels (r = 0.53, P < 0.01).Conclusion. Serum IL-12 levels in patients with far-advanced gastric cancer were significantly lower than those in patients with less-advanced gastric cancer. This is because macrophages in patients with far-advanced cancer would be hectic and unable to produce sufficient IL-12.