Aromatase inhibitor anastrozole modifies cellular functions in gingival fibroblasts and endothelial cells: possible periodontal complications of aromatase inhibitor treatment
Aromatase inhibitor anastrozole modifies cellular functions in gingival fibroblasts and endothelial cells: possible periodontal complications of aromatase inhibitor treatment
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芳香酶抑制剂阿那曲唑改变牙龈成纤维细胞和内皮细胞的细胞功能：芳香酶抑制剂治疗可能出现的牙周并发症
DOI：
10.1111/jre.12881
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发表时间：
2021
影响因子：
3.5
通讯作者：
Shinya Murakami
中科院分区：
文献类型：
--
作者：
Shiori Hasegawa Manabu Yanagita Mari Tatsumi Motozo Yamashita Masahiro Kitamura;Shinya Murakami
背景最近的研究表明，芳香酶抑制剂治疗会导致牙周炎患病率增加。目的在本研究中，我们评估了芳香酶抑制剂阿那曲唑对人牙龈成纤维细胞（HGF）和内皮细胞细胞功能的影响。方法检测了胶原蛋白、细胞外基质（ECM）蛋白、基质金属蛋白酶（MMP）和金属蛋白酶组织抑制剂（TIMP）的表达水平暴露于阿那曲唑的 HGF 中。此外，在牙龈卟啉单胞菌脂多糖存在的情况下评估了用阿那曲唑培养的HGF的炎症反应。我们还评估了暴露于阿那曲唑的内皮细胞的血管通透性和血管内皮（VE）钙粘蛋白表达。结果阿那曲唑增强了 HGF 中胶原蛋白、ECM 蛋白、TIMP 和炎症细胞因子的表达水平，以及内皮细胞的血管通透性。此外，阿那曲唑降低了内皮细胞中 HGF 和 VE-钙粘蛋白中 MMP 的表达水平。结论这些结果表明阿那曲唑调节 HGF 和内皮细胞中的各种细胞功能。
BackgroundRecent studies have shown that treatment with aromatase inhibitors contributes to an increased prevalence of periodontitis.ObjectiveIn this study, we assessed effects of the aromatase inhibitor anastrozole on cellular function of human gingival fibroblasts (HGFs) and endothelial cells.MethodsExpression levels of collagen, extracellular matrix (ECM) proteins, matrix metalloproteinases (MMPs), and tissue inhibitors of metalloproteinases (TIMPs) were examined in HGFs exposed to anastrozole. Furthermore, inflammatory responses in HGFs cultured with anastrozole were evaluated in the presence ofPorphyromonas gingivalislipopolysaccharide. We also evaluated the vascular permeability and vascular endothelial (VE)‐cadherin expression of endothelial cells exposed to anastrozole.ResultsAnastrozole enhanced expression levels of collagen, ECM proteins, TIMPs, and inflammatory cytokines in HGFs, as well as vascular permeability of endothelial cells. In addition, anastrozole reduced expression levels of MMPs in HGFs and VE‐cadherin in endothelial cells.ConclusionThese results suggest that anastrozole modulates various cellular functions in HGFs and endothelial cells.