Human immunity to Pseudomonas aeruginosa. I. In-vitro interaction of bacteria, polymorphonuclear leukocytes, and serum factors.
Human immunity to Pseudomonas aeruginosa. I. In-vitro interaction of bacteria, polymorphonuclear leukocytes, and serum factors.
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人体对铜绿假单胞菌的免疫力。
DOI：
10.1093/infdis/126.3.257
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发表时间：
1972
期刊：
影响因子：
--
通讯作者：
Donald Armstrong
中科院分区：
文献类型：
--
作者：
Lowell S. Young;Donald Armstrong
用血清杀菌试验、吞噬和杀伤定量试验评价铜绿假单胞菌与人多形核白细胞(PMN)和血清因子的相互作用。在46株血培养铜绿假单胞菌中，有42株(91%)对血清有抵抗力；从12名从菌血症中幸存下来的患者中回收的微生物没有被含有高滴度抗体的新鲜自体疗养血清杀死。然而，来自这些患者的新鲜血清(以及新鲜的正常血清)，当与正常的PMN结合时，在60-120分钟内促进对细菌的10-100倍杀灭。中性粒细胞的最佳吞噬作用需要热稳定抗体和热不稳定调色素的存在；后者的活性主要是由于补体的前四个成分。细菌感染组存活者血清中热稳定调理抗体显著增强。正常运作的PMN对宿主防御至关重要，因为大多数侵袭性铜绿假单胞菌菌株对血清具有抵抗力。
The interaction between Pseudomonas aeruginosa, human polymorphonuclear leukocytes (PMNs), and serum factors was evaluated by use of serum bactericidal tests and quantitative tests of phagocytosis and killing. Forty-two of 46 blood-culture isolates (91%) of P. aeruginosa were serum-resistant; organisms recovered from 12 patients who survived bacteremia were not killed by fresh autologous convalescent serum containing high titers of antibody. However, fresh sera from these patients (as well as fresh normal sera), when combined with normal PMNs, promoted a 10to 100-fold killing of bacteria within 60-120 min. Optimal phagocytosis by PMNs required the presence of heat-stable antibody and heat-labile opsonin; the activity of the latter was primarily due to the first four components of complement. Heat-stable opsonizing antibody was greatly augmented in sera of survivors of bacteremic infection. Normally functioning PMNs are essential to host defenses, since most invasive strains of P. aeruginosa are resistant to serum.