Structure-function correlation of human programmed cell death 5 protein.
Structure-function correlation of human programmed cell death 5 protein.
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DOI：
10.1016/j.abb.2009.03.018
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发表时间：
2009-06
影响因子：
3.9
通讯作者：
H. Yao;Lanjun Xu;Yingang Feng;Dongsheng Liu;Yingyu Chen;Jinfeng Wang
中科院分区：
文献类型：
--
作者：
H. Yao;Lanjun Xu;Yingang Feng;Dongsheng Liu;Yingyu Chen;Jinfeng Wang
人程序性细胞死亡5（PDCD 5）是一种转位蛋白，在细胞凋亡过程中起重要作用。虽然关于PDCD 5的功能有积累的数据，但尚未研究PDCD 5的结构与功能的相关性。本文采用多维核磁共振方法、流式细胞仪和荧光显微镜对PDCD 5的结构与功能关系进行了研究。完整的PDCD 5的三维结构和PDCD 5的内部运动已经确定。PDCD 5具有低柔性的紧凑的核心结构，在N-末端区域具有两个移动的α-螺旋和柔性的非结构化C-末端区域。流式细胞术和不同PDCD 5片段的内化测量表明，带电荷的残基对蛋白的促凋亡和细胞易位能力至关重要。综合分析揭示了一个事实，即似乎最参与功能的区域在PDCD 5中也更灵活。
Human programmed cell death 5 (PDCD5) is a translocatory protein playing an important role in the apoptotic process of cells. Although there are accumulated data about PDCD5 function, the correlation of the structure with the function of PDCD5 has not been investigated. Here, we report the studies of structure–function relationship of PDCD5 by multidimensional NMR methods and by FACScan flow cytometer and fluorescence microscope. The 3D structure of intact PDCD5 and the internal motions of PDCD5 have been determined. PDCD5 has a compact core structure of low flexibility with two mobile α-helices at N-terminal region and a flexible unstructured C-terminal region. The flow cytometry and internalization measurements of different PDCD5 fragments indicate that the charged residues are crucial for the ability of apoptosis-promoting and cell translocation of the protein. Combined analyses reveal a fact that the regions that seem to be most involved in the function also are more flexible in PDCD5.