Modeling of Mitochondrial Donut Formation
Modeling of Mitochondrial Donut Formation
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DOI：
10.1016/j.bpj.2015.07.039
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发表时间：
2015-09-01
影响因子：
3.4
通讯作者：
Liu, Xingguo
中科院分区：
文献类型：
--
作者：
Long, Qi;Zhao, Danyun;Liu, Xingguo
线粒体是高度动态的细胞器。融合和分裂的连续循环在线粒体代谢和细胞信号传导中起重要作用。以前，一种新的线粒体形态，甜甜圈，在缺氧-复氧或渗透压变化后的细胞中报道。然而，甜甜圈形成的机制仍然难以捉摸。在这里，我们获得了甜甜圈直径的分布（D = 2 R），发现95%>0.8 mm。我们还使用超分辨率和电子显微镜对线粒体小管直径进行了高精度测量。然后，我们通过计算线粒体弯曲能和渗透势建立了一个模型，在甜甜圈形成。结果表明，在圆环成形过程中，弯曲能随曲率半径R的减小而增大，尤其是曲率半径R越小，弯曲能越大
Mitochondria are highly dynamic cell organelles. Continual cycles of fusion and fission play an important role in mitochondrial metabolism and cellular signaling. Previously, a novel mitochondrial morphology, the donut, was reported in cells after hypoxia-reoxygenation or osmotic pressure changes. However, the mechanism of donut formation remained elusive. Here, we obtained the distribution of donut diameters (D = 2R) and found that 95% are >0.8 mm. We also performed highly precise measurements of the mitochondrial tubule diameters using superresolution and electron microscopy. Then, we set up a model by calculating the mitochondrial bending energy and osmotic potential during donut formation. It shows that the bending energy is increased as the radius of curvature, R, gets smaller in the process of donut formation, especially for radii