Induction of intestinal rotavirus-specific antibodies in respiratory, but not gut, lymphoid tissues following mucosal immunization of mice with inactivated rotavirus.
Induction of intestinal rotavirus-specific antibodies in respiratory, but not gut, lymphoid tissues following mucosal immunization of mice with inactivated rotavirus.
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用灭活的轮状病毒对小鼠进行粘膜免疫后，在呼吸道而非肠道淋巴组织中诱导肠道轮状病毒特异性抗体。
DOI：
10.1006/viro.2001.1180
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Clark,SL
中科院分区：
文献类型：
--
作者：
Coffin,SE;Clark,SL
鼻内（i.n.），而非口服，用灭活的轮状病毒免疫小鼠诱导了对抗攻击的保护。为了了解i.n.用灭活的轮状病毒免疫引起保护性免疫，我们检查了轮状病毒特异性B细胞活化的位点和i. n.用灭活的恒河猴轮状病毒接种小鼠。我们发现（1）i.n.，但不是口服，接种诱导攻击后的部分保护;（2）i.n.，经口接种后，肠道淋巴组织产生轮状病毒特异性IgM、IgA和IgG，而经口接种后，肠道淋巴组织产生轮状病毒特异性IgM、IgA和IgG。接种后，鼻相关淋巴组织（NALT）和支气管淋巴结（BLN）是轮状病毒特异性抗体的初始产生部位。这些研究表明，接种灭活轮状病毒后，与肠道淋巴组织相比，呼吸道组织中的病毒特异性效应B细胞可能更容易被激活。需要进一步的研究来确定这些观察结果是否是由于NALT和BLN的微环境与派尔集合淋巴结相比的根本差异，或者是呼吸道和胃肠道之间的解剖差异的函数。
Intranasal (i.n.), but not oral, immunization of mice with inactivated rotavirus induces protection against challenge. To understand the mechanisms by which i.n. immunization with inactivated rotavirus evokes protective immunity, we examined the site of rotavirus-specific B cell activation and the origins of intestinal IgA-secreting B cells following i.n. inoculation of mice with inactivated rhesus rotavirus. We found that (1) i.n., but not oral, inoculation induced partial protection after challenge; (2) i.n., but not oral, inoculation induced production of rotavirus-specific IgM, IgA, and IgG by intestinal lymphoid tissues; and (3) after i.n. inoculation, nasal-associated lymphoid tissues (NALT) and bronchial lymph nodes (BLN) were the sites of initial production of rotavirus-specific antibodies. These studies indicate that after inoculation with inactivated rotavirus, virus-specific effector B cells may be more easily activated in respiratory, compared to intestinal, lymphoid tissues. Additional studies are needed to determine if these observations are due to fundamental differences in the microenvironment of NALT and BLN compared to Peyer's patches or are a function of the anatomic differences between the respiratory and the gastrointestinal tracts.