SspA positively controls exopolysaccharides production and biofilm formation by up-regulating the algU expression in Pseudoalteromonas sp. R3.
SspA positively controls exopolysaccharides production and biofilm formation by up-regulating the algU expression in Pseudoalteromonas sp. R3.
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DOI：
10.1016/j.bbrc.2020.09.118
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发表时间：
2020-09
影响因子：
3.1
通讯作者：
Zhiliang Yu;Jiadi Zhang;Mengdan Ding;Shijun Wu;Shuangjia Li;Mengting Zhang;Jianhua Yin;Qiu Meng-
中科院分区：
文献类型：
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作者：
Zhiliang Yu;Jiadi Zhang;Mengdan Ding;Shijun Wu;Shuangjia Li;Mengting Zhang;Jianhua Yin;Qiu Meng-
生物膜的形成增强了微生物对环境胁迫的响应的存活和持久性。严格饥饿蛋白A（SspA）是细菌在环境胁迫下生存的重要调节因子。然而，SspA和生物膜形成之间的联系基本上还不清楚。在这项研究中，我们提出的证据表明，SspA积极控制生物膜的形成，通过上调胞外多糖（EPS）的生产海洋细菌Pseudoalteromonas sp. R3。qPCR和lacZ报告系统均一致地显示SspA正调控EPS生物合成基因簇的表达。SspA通过抑制组蛋白样核苷酸结构蛋白（H-NS）基因的表达来调节细菌的生理功能，而SspA对假交替单胞菌（Pseudoalteromonassp.R3）胞外多糖的产生和生物膜形成的作用不依赖于H-NS。相反，SspA正调控sigma因子AlgU编码基因的表达，从而影响EPS生物合成和生物膜形成。鉴于SspA在生物膜形成中的重要作用，我们认为，对海洋环境胁迫的耐受性的提高可能与SspA参与的生物膜形成的调谐有关。
Biofilm formation enhances the survival and persistence of microorganisms in response to environmental stresses. It has been revealed that stringent starvation protein A (SspA) can function as an important regulator dealing with environmental stresses for bacterial survival. However, the connection between SspA and biofilm formation is essentially unclear yet. In this study, we presented evidence showing SspA positively controls biofilm formation by up-regulating exopolysaccharides (EPS) production in marine bacterium Pseudoalteromonas sp. R3. Both qPCR andlacZreporter system congruously revealed that SspA positively controls the expression of EPS biosynthesis gene cluster. Unlike generally accepted thought that SspA regulates bacterial physiology by inhibiting the expression of histone-like nucleotide structuring protein (H-NS) gene, the function of SspA on EPS production and biofilm formation in Pseudoalteromonas sp. R3 is H-NS-independent. Instead, SspA positively regulates the expression of sigma factor AlgU-encoding gene, thus affecting EPS biosynthesis and biofilm formation. In view of the important role of SspA in biofilm formation, we believe that the improvement of tolerance to marine environmental stresses could be related to tuning of SspA-involved biofilm formation.