Targeted plasma metabolome response to variations in dietary glycemic load in a randomized, controlled, crossover feeding trial in healthy adults.
Targeted plasma metabolome response to variations in dietary glycemic load in a randomized, controlled, crossover feeding trial in healthy adults.
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DOI：
10.1039/c5fo00287g
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发表时间：
2015-09
期刊：
影响因子：
6.1
通讯作者：
Lampe JW
中科院分区：
文献类型：
--
作者：
Barton S;Navarro SL;Buas MF;Schwarz Y;Gu H;Djukovic D;Raftery D;Kratz M;Neuhouser ML;Lampe JW
低血糖负荷与高血糖负荷（GL）饮食模式与肥胖和慢性疾病（如癌症和心血管疾病）呈负相关。这些关联持续存在，超出了单独增加纤维所提供的保护，代表了我们对GL代谢作用的理解中的一个重要空白。我们进行了一项随机、对照、交叉喂养试验，分为高GL和低GL两个28天的饮食期。使用LC-MS，对19名18-45岁健康成人的血浆样本进行了155种代谢物的靶向代谢组学分析。14种代谢物在饲料之间存在显著差异（P<0.05），其中犬尿氨酸在Bonferroni校正后仍具有显著性（P<4×10-4）。丰度差异最大的代谢物是犬尿氨酸和三甲胺-N-氧化物（TMAO），在食用低GL饮食后两者均显著升高。偏最小二乘判别分析表明，两种饮食之间的明确分离，但没有特定的途径，确定在途径分析。我们发现14种血浆代谢物存在显著差异，表明对低和高GL饮食的代谢反应不同。犬尿酸盐与炎症减少有关，可能是低GL饮食的保护作用表现出来的一种机制，需要进一步评价。本试验在clinicaltrials.gov上注册为NCT 00622661。
Low versus high glycemic load (GL) diet patterns are inversely associated with obesity and chronic diseases such as cancer and cardiovascular disease. These associations persist beyond the protection afforded by increased fiber alone, representing an important gap in our understanding of the metabolic effects of GL. We conducted a randomized, controlled, crossover feeding trial of two 28-day diet periods of high and low GL. Using LC-MS, targeted metabolomics analysis of 155 metabolites was performed on plasma samples from 19 healthy adults aged 18-45 years. Fourteen metabolites differed significantly between diets (P<0.05), with kynurenate remaining significant after Bonferroni correction (P<4×10-4). Metabolites with the largest difference in abundance were kynurenate and trimethylamine-N-oxide (TMAO), both significantly higher after consumption of the low GL diet. Partial least squares-discriminant analysis showed clear separation between the two diets; however no specific pathway was identified in pathway analyses. We found significant differences in 14 plasma metabolites suggesting a differing metabolic response to low and high GL diets. Kynurenate is associated with reduced inflammation, and may be one mechanism through which protective effects of a low GL diet are manifested and warrants further evaluation. This trial was registered at clinicaltrials.gov as NCT00622661.