Continuous and Batch Production of Chloroperoxidase by Mycelial Pellets of Caldariomyces fumago in an Airlift Fermentor
Continuous and Batch Production of Chloroperoxidase by Mycelial Pellets of Caldariomyces fumago in an Airlift Fermentor
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在气升式发酵罐中利用烟热霉菌丝体颗粒连续批量生产氯过氧化物酶
DOI：
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发表时间：
1989
影响因子：
4.4
通讯作者：
M. Pickard
中科院分区：
文献类型：
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作者：
R. Carmichael;M. Pickard
用气升式发酵罐研究了细胞外血红素糖蛋白氯过氧化物酶（CPO）的批量和连续生产。在1升发酵罐中培养前，先用少量接种物诱导富马Caldariomyces fumago CMI 89362形成微球。连续更换果糖盐培养基（稀释率为0.008 h−1），CPO的平均浓度为128±10 mg l−1，持续8天。当稀释率低于0.033 h - 1时，最佳CPO产率平均为1.2±0.1 mg CPO h - 1。改变饲料溶液的碳水化合物含量或开始饲喂的时间对CPO的产生量没有显著影响。在气升式发酵罐中分批发酵产生的CPO浓度最高为280±80 mg CPO l - 1，尽管有两次CPO浓度达到400至450 mg l - 1，这是摇瓶培养中自由菌丝获得量的两倍。
Batch and continuous production of the extracellular heme glycoprotein chloroperoxidase (CPO) was studied with an airlift fermentor. We induced Caldariomyces fumago CMI 89362 to form pellets by transferring a small inoculum volume in preculture prior to growth in a 1-liter fermentor. Continuous replacement of the fructose-salts medium (dilution rate, 0.008 h−1) supported continuous CPO formation at an average concentration of 128 ± 10 mg of CPO liter−1 for 8 days. Optimum CPO production rates averaged 1.2 ± 0.1 mg of CPO h−1 at dilution rates below 0.033 h−1. Varying the carbohydrate content of the feed solution or the time of starting the feed did not significantly alter the amount of CPO produced. Batch fermentation in the airlift fermentor resulted in maximum CPO concentrations of 280 ± 80 mg of CPO liter−1, although on two separate occasions CPO concentrations reached 400 to 450 mg liter−1, which was double the amount obtained by free hyphae in shake flask culture.