THE DROSOPHILA POLYCOMB-GROUP GENE ENHANCER OF ZESTE CONTAINS A REGION WITH SEQUENCE SIMILARITY TO TRITHORAX
THE DROSOPHILA POLYCOMB-GROUP GENE ENHANCER OF ZESTE CONTAINS A REGION WITH SEQUENCE SIMILARITY TO TRITHORAX
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DOI：
10.1128/mcb.13.10.6357
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发表时间：
1993-10-01
影响因子：
5.3
通讯作者：
GELBART, WM
中科院分区：
文献类型：
--
作者：
JONES, RS;GELBART, WM
作为典型的Polycomb-group位点，zeste [E(z)]基因的增强子负调控Antennapedia （ANT-C）和Bithorax （BX-C）基因复合物的片段识别基因。第二类基因座，统称为三胸组，作为ANT-C和BX-C基因的正向调节因子起拮抗作用。E(z)基因的分子分析预测了一个760个氨基酸的蛋白质产物。E(z)羧基末端附近的116个氨基酸区域与三胸蛋白的羧基末端区域相同41.2%（相似68.4%）。三胸蛋白的这一部分是先前显示与急性白血病相关的人类蛋白（ALL-1/Hrx）具有广泛同源性的更大区域的一部分。在这116个氨基酸中，E(z)和ALL-1/Hrx有43.9%相同（68.4%相似）。除此之外，E(z)与之前描述的任何蛋白质都没有明显的相似之处。由于这一序列相似性区域由两种具有拮抗功能的蛋白质共享，我们认为它可能包含一个与共同目标（核酸或蛋白质）相互作用的结构域。对转录的相反影响可能由这两种蛋白质的其他部分决定。
As is typical of Polycomb-group loci, the Enhancer of zeste [E(z)] gene negatively regulates the segment identity genes of the Antennapedia (ANT-C) and Bithorax (BX-C) gene complexes. A second class of loci, collectively known as the trithorax group, plays an antagonistic role as positive regulators of the ANT-C and BX-C genes. Molecular analysis of the E(z) gene predicts a 760-amino-acid protein product. A region of 116 amino acids near the E(z) carboxy terminus is 41.2% identical (68.4% similar) with a carboxy-terminal region of the trithorax protein. This portion of the trithorax protein is part of a larger region previously shown to share extensive homology with a human protein (ALL-1/Hrx) that is implicated in acute leukemias. Over this same 116 amino acids, E(z) and ALL-1/Hrx are 43.9% identical (68.4% similar). Otherwise, E(z) is not significantly similar to any previously described proteins. As this region of sequence similarity is shared by two proteins with antagonistic functions, we suggest that it may comprise a domain that interacts with a common target, either nucleic acid or protein. Opposite effects on transcription might then be determined by other portions of the two proteins.