Characterization of a rotavirus rearranged gene 11 by gene reassortment
Characterization of a rotavirus rearranged gene 11 by gene reassortment
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DOI：
10.1007/s007050050411
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发表时间：
1998-01-01
影响因子：
2.7
通讯作者：
Spencer, E
中科院分区：
文献类型：
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作者：
Chnaiderman, J;Diaz, J;Spencer, E
研究了用从猪轮状病毒株获得的携带头尾重复的基因替换轮状病毒SA-Ⅱ的基因Ii的效果。具有重复基因的猪轮状病毒株（CC 86）在共感染时表现出允许其他病毒株过度生长的表型，并且在CV-1和MA-104猴肾细胞中接种时表现出增加的空斑大小。利用重配方法，将猪轮状病毒的重复基因引入SA-11病毒中，取代常规基因II。对重新鉴定的菌株进行了表征，以找出其他病毒基因片段的来源。基于电泳迁移率，区段1、2、3、5、7、8和10被鉴定为SA-11。区段4、6和9的SA-11来源通过用多克隆和单克隆抗体中和以及通过ELISA来确认。结果表明，该毒株是一株携带SA-11基因的单克隆毒株，但SA-11基因的复制基因来源于猪病毒CC 86。在体内复制和合成病毒蛋白的能力是相同的，在重申的病毒和亲本菌株。序列分析表明，这种新的表型不是由基因II的重复引起的，而是由NSP 5基因编码区的突变引起的。
The effect of replacement of gene Ii of rotavirus SA-II by a gene carrying a head to tail duplication obtained from a swine rotavirus strain was studied. The swine rotavirus strain with a duplicated gene (CC86) exhibits both a phenotype that allows to overgrow other viral strains when coinfected and an increased plaque size when plated in both CV-1 and MA-104 monkey kidney cells. Using reassortment methods the duplicated gene of the swine rotavirus was introduced into the SA-11 virus, replacing the regular gene II. The reasserted strain was characterized to find out the origin of each of the other viral gene segments. Based on electrophoretic mobilities segments 1, 2, 3, 5, 7, 8 and 10 were identified as of SA-11. The SA-11 origin of the segments 4, 6 and 9 was confirmed by neutralization with polyclonal and monoclonal antibodies and by ELISA. The results suggest that the new reassortant virus was a monoreassortant carrying SA-11 genes except the duplicated gene originated from the swine virus CC86. The ability to in vivo replicate and to synthesize viral proteins was identical in the reasserted virus and the parental strains. Sequence analysis indicates that the new phenotype does not originate in the duplication of gene Il but possibly from mutations in the coding region of NSP5 gene that may result in different phosphorylation patterns of the protein.