Multiplex targeting, tracking, and imaging of apoptosis by fluorescent surface enhanced raman spectroscopic dots
Multiplex targeting, tracking, and imaging of apoptosis by fluorescent surface enhanced raman spectroscopic dots
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DOI：
10.1021/bc070011i
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发表时间：
2007-07-01
影响因子：
4.7
通讯作者：
Cho, Myung-Haing
中科院分区：
文献类型：
--
作者：
Yu, Kyeong Nam;Lee, Sang-Myung;Cho, Myung-Haing
我们开发了多功能荧光表面增强拉曼光谱标记材料（F - SERS点），它由嵌入银纳米粒子的二氧化硅球组成，含有荧光有机染料和特定的拉曼标记，用于对生物体内细胞/分子事件进行多重靶向、追踪和成像。在这项研究中，用特定的靶向抗体（BAX和BAD）制备的F - SERS点被用于检测细胞凋亡。F - SERS点在几种细胞系中未显示出任何特殊毒性。F - SERS点能够通过荧光图像以及拉曼信号在细胞和组织中高效且同时地对细胞凋亡进行监测，具有高选择性。我们的研究结果清楚地表明，F - SERS点可轻松应用于对维持细胞内稳态至关重要的多种细胞/分子事件的多重分析。
We have developed multifunctional fluorescent surface enhanced Raman spectroscopic tagging material (F-SERS dots) composed of silver nanoparticle-embedded silica spheres with fluorescent organic dye and specific Raman labels for multiplex targeting, tracking, and imaging of cellular/molecular events in the living organism. In this study, F-SERS dots fabricated with specific target antibodies (BAX and BAD) were employed for the detection of apoptosis. The F-SERS dots did not show any particular toxicity in several cell lines. The F-SERS dots could monitor the apoptosis effectively and simultaneously through fluorescent images as well as Raman signals in both cells and tissues with high selectivity. Our results clearly demonstrate that F-SERS dots can be easily applicable to multiplex analysis of diverse cellular/molecular events important for maintaining cellular homeostasis.